Reduction of the argyrophilic nucleolar organizing region associated protein synthesis with age in buccal epithelial cells of healthy individuals

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• 作者：Burak Selvi ; Halil Demirtas ; Recep Eroz…
• 关键词：NOR ; AgNORs ; Buccal epithelial cells ; rDNA ; Nucleolus
• 刊名：Aging Clinical and Experimental Research
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：27
• 期：2
• 页码：201-208
• 全文大小：481 KB
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• 刊物主题：Geriatrics/Gerontology;
• 出版者：Springer International Publishing
• ISSN：1720-8319

文摘

Background Nucleolus organizer regions (NORs) consist of the rRNA coding gene family (rDNA) in the cell nucleus. The argyrophilic proteins are selectively stained with silver nitrate and bind these regions. It was reported that NOR (rDNA) activity decreases in human lymphocytes, fibroblasts and bone marrow with age. However, to our knowledge there have not been any studies related to the NORs in oral epithelial cells of healthy individuals. Aim Our aim is to detect any correlation between age and Total AgNOR area/Total nucleus area (TAA/TNA) values in buccal epithelial cells of healthy individuals. Methods Oral epithelial cells from 50 healthy individuals (age range of 2-0?years old) were spread onto a clean glass slide, air dried and fixed. Then the AgNOR staining protocol was performed on these cells. TAA/TNA ratio and AgNOR dots were calculated using software. From each person 50 oral epithelial cells were evaluated. Results Statistically significant correlations were found between mean TAA/TNA values and age (Rsq?=?0.534, p?p?p?p? Conclusion There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.