Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture

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• 作者：Franciele Osmarini Lunardi ; Roberta Nogueira Chaves…
• 关键词：Vitrification ; Ovarian fragments ; Isolated secondary follicle ; Nucleolus organizer regions ; In vitro culture
• 刊名：Cell and Tissue Research
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：362
• 期：1
• 页码：241-251
• 全文大小：1,382 KB
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• 作者单位：Franciele Osmarini Lunardi (1)
  Roberta Nogueira Chaves (2)
  Laritza Ferreira de Lima (1)
  Valdevane Rocha Ara煤jo (1)
  Ivina Rocha Brito (1)
  Carlos Eduardo Azevedo Souza (3)
  Mariana Arag茫o Matos Donato (4)
  Christina Alves Peixoto (4)
  Andras Dinnyes (5)
  Cl谩udio Cabral Campello (1)
  Jos茅 Ricardo de Figueiredo (1)
  Ana Paula Ribeiro Rodrigues (1)
  
  1. Laboratory of Manipulation of Oocytes and Ovarian Pre-antral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Cear谩 State University, Fortaleza, CE, Brazil
  2. Health Center, University of Fortaleza (UNIFOR), Edson Queiroz, Fortaleza, Cear谩, Brazil
  3. State University of Ceara, Fortaleza, Ceara, Brazil
  4. Laborat贸rio de Ultraestrutura, CPqAM-FIOCRUZ, Universidade Federal de Pernambuco, Recife, PE, Brazil
  5. Molecular Animal Biotechnology Laboratory, Szent Istvan University, G枚d枚ll枚, Hungary
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

The risk of reintroducing malignant cells after ovarian graft into patients following post-cancer treatment is an obstacle for clinical applications (autotransplantation). In this context, in vitro follicle culture would be an alternative to transplantation in order to minimize such risks. Therefore, the aim of this study was to compare the development of secondary follicles after vitrification in isolated form (without stroma) with vitrification in in situ form (within fragments of ovarian tissue). Follicles were first isolated from ovarian fragments from mixed-breed ewes and then vitrified; these comprised the Follicle-Vitrification group (Follicle-Vit), or fragments of ovarian tissue were first vitrified, followed by isolation of the follicles, resulting in the Tissue-Vitrification group (Tissue-Vit). Control and vitrified groups were submitted to in vitro culture (6 days) and follicular morphology, viability, antrum formation, follicle and oocyte diameter, growth rate, ultrastructural characteristics and cell proliferation were evaluated. The percentages of morphologically normal follicles and antrum formation were similar among groups. Follicular viability and oocyte diameter were similar between Follicle-Vit and Tissue-Vit. The follicular diameter and growth rate of Follicle-Vit were similar to the Control, while those of Tissue-Vit were significantly lower compared to the Control. Both vitrified groups had an augmented rate of granulosa cellular proliferation compared to Control. Secondary follicles can be successfully vitrified before or after isolation from the ovarian tissue without impairing their ability to survive and grow during in vitro culture. Keywords Vitrification Ovarian fragments Isolated secondary follicle Nucleolus organizer regions In vitro culture