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作者单位：Ana Carolina Coelho (1) (3)
Thais Lemos de Mattos (1)
Patrik Viana (1)
Maria Leandra Terencio (2)
Carlos Henrique Schneider (3)
Marcelo Menin (3)
Maria Claudia Gross (3)

1. Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Manaus, AM, 69080-971, Brazil
3. Laboratório de Citogenômica, Departamento de Biologia, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Av. General Rodrigo Octávio Jordão Ramos, 3000, Manaus, AM, 69077-000, Brazil
2. Universidade Federal da Integração Latino-Americana, Av. Silvio Américo Sasdelli, 1842 - Vila A, Foz do Iguaçu, PR, CEP 85866-000, Brazil

刊物类别：Biomedical and Life Sciences

刊物主题：Life Sciences
Life Sciences
Animal Genetics and Genomics
Plant Genetics and Genomics
Human Genetics
Microbial Genetics and Genomics

出版者：Springer Netherlands

ISSN：1573-6857

文摘

The genera Leptodactylus and Adenomera comprise 92 species distributed throughout the Neotropical region. These species have a modal diploid chromosome number 2n = 22. However, chromosome rearrangements are evident in the differentiation of five intra-generic groups in the genus Leptodactylus (L. fuscus, L. latrans, L. marmoratus (formally composed by the species of the genus Adenomera), L. melanonotus, L. pentadactylus), yet it is not clear if there is a karyotype pattern for each group. Aiming to understand the intra-generic and interspecific karyotype patterns of Leptodactylus and Adenomera, cytogenetic analyses were performed in A. andreae, L. macrosternum, L. pentadactylus, L. petersii, and L. riveroi using conventional staining, C-banding, nucleolus organizer region (NOR) and hybridization in situ fluorescent (FISH). The karyotype of Leptodactylus riveroi was described for the first time. Adenomera andreae had 2n = 26, while the remaining species 2n = 22. The NOR was found on pair No. 8 of A. andreae, L. macrosternum, L. pentadactylus, and L. riveroi, whereas L. petersii had it on pairs Nos. 6 and 10. These locations were confirmed by the FISH with 18S rDNA probe, except for pair No. 10 of L. petersii. The C-banding pattern was evident at the centromeres of chromosomes of all species and some interspecific variations were also observed. 2n = 22 was observed in the species of the L. latrans group, as well as in the intra-generic groups L. fuscus and L. pentadactylus; in the L. melanonotus group there were three diploid chromosome numbers 2n = 20, 22 and 24; and a larger variation in 2n was also evident in the L. marmoratus group. Keywords Leptodactylid diversity Anuran cytotaxonomy Chromosome banding rDNA FISH NOR phenotypes