CsrA impacts survival of Yersinia enterocolitica by affecting a myriad of physiological activities

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• 作者：Karen LeGrand (1) (2)
  Shane Petersen (2)
  Yan Zheng (2) (3)
  Kang K Liu (2)
  Gulustan Ozturk (2)
  Jing-Yu Chen (2) (4)
  Glenn M Young (1) (2)
  
  1. Microbiology Graduate Group ; University of California ; Davis ; CA ; USA
  2. Department of Food Science and Technology ; University of California ; Davis ; Davis ; CA ; USA
  3. College of Food Science ; Shenyang Agricultural University ; Shenyang ; PR China
  4. College of Food Science and Nutritional Engineering ; China Agricultural University ; Beijing ; China
  
• 关键词：Yersinia ; CsrA ; Csr system ; Motility ; Salt sensitivity ; Antibiotic sensitivity ; Temperature sensitivity ; Psychrotroph ; Mutant selection
• 刊名：BMC Microbiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：820 KB
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• 刊物主题：Microbiology; Biological Microscopy; Fungus Genetics; Parasitology; Virology; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1471-2180

文摘

Background A previous study identified a Yersinia enterocolitica transposon mutant, GY448, that was unable to export the flagellar type three secretion system (T3SS)-dependent phospholipase, YplA. This strain was also deficient for motility and unable to form colonies on Lauria-Bertani agar medium. Preliminary analysis suggested it carried a mutation in csrA. CsrA in Escherichia coli is an RNA-binding protein that is involved in specific post-transcriptional regulation of a myriad of physiological activities. This study investigated how CsrA affects expression of the flagellar regulatory cascade that controls YplA export and motility. It also explored the effect of csrA mutation on Y. enterocolitica in response to conditions that cue physiological changes important for growth in environments found both in nature and the laboratory. Results The precise location of the transposon insertion in GMY448 was mapped within csrA. Genetic complementation restored disruptions in motility and the YplA export phenotype (Yex), which confirmed this mutation disrupted CsrA function. Mutation of csrA affected expression of yplA and flagellar genes involved in flagellar T3SS dependent export and motility by altering expression of the master regulators flhDC. Mutation of csrA also resulted in increased sensitivity of Y. enterocolitica to various osmolytes, temperatures and antibiotics. Conclusions The results of this study reveal unique aspects of how CsrA functions in Y. enterocolitica to control its physiology. This provides perspective on how the Csr system is susceptible to adaptation to particular environments and bacterial lifestyles.