Optimization study of 2-hydroxyquinoxaline (2-HQ) biodegradation by Ochrobactrum sp. HQ1

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• 作者：G. V. Subba Reddy ; M. Md. Rafi ; S. Rubesh Kumar ; N. Khayalethu…
• 关键词：2 ; Hydroxyquinoxaline ; Ochrobactrum sp. HQ1 ; 16S rRNA sequence analysis ; HPLC and GC–MS analysis
• 刊名：3 Biotech
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：1
• 全文大小：879 KB
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• 作者单位：G. V. Subba Reddy (1) (2)
  M. Md. Rafi (3)
  S. Rubesh Kumar (4)
  N. Khayalethu (2)
  D. Muralidhara Rao (3)
  B. Manjunatha (5)
  G. H. Philip (3)
  B. R. Reddy (1)
  
  1. Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu-, 515003, Andhra Pradesh, India
  2. Department of Agriculture and Animal Health, University of South Africa, Private Bag X6, Florida, 1710, Johannesburg, South Africa
  3. Department of Biotechnology, Sri Krishnadevaraya University, Anantapuramu-, 515003, Andhra Pradesh, India
  4. Department of Pharmaceutical Analysis, JNTUA-Oil Technological Research Institute, Anantapuramu-, 515001, Andhra Pradesh, India
  5. Department of Life Sciences, Universidad de las Fuerzas Armadas-ESPE, Sangolquí, Quito, Ecuador, South America
  
• 刊物主题：Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-5738

文摘

A novel aerobic gram-negative bacterial strain capable of utilizing 2-hydroxyquinoxaline (2-HQ) as sole source of carbon and energy was isolated from Indian agricultural soil and named as HQ1. Strain HQ1 was identified as Ochrobactrum sp. on the basis of morphology, physico-biochemical characteristics and 16S rRNA sequence analysis. The generation time of Ochrobactrum sp. HQ1 on 2-HQ at log phase is 0.71 h or 42.6 min. The degradation of 2-HQ by HQ1 under various physico-chemical parameters was analysed by HPLC and observed to be optimum with a high inoculum density (1.0 OD) at pH 7–8, temperatures 37–40°C and a high concentration of 2-HQ (500 ppm). Degradation of 2-HQ was also improved when additional nitrogen sources were used and this was attributed to the enhanced growth of the bacterium on the readily available nitrogen sources. Analysis of 2-HQ degradation by GC–MS resulted in elucidation of the degradation pathway for HQ1, a novel observation for aerobic Gram-negative bacteria. These findings are a possible indication of the application of HQ1 in the bioremediation of pesticide/metabolite contamination. Keywords 2-Hydroxyquinoxaline Ochrobactrum sp. HQ1 16S rRNA sequence analysis HPLC and GC–MS analysis