Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus

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• 作者：Maria Assunta Biscotti ; Marco Barucca ; Teresa Capriglione ; Gaetano Odierna ; Ettore Olmo and Adriana Canapa
• 关键词：chiton ; fluorescence in ; situ hybridization ; Nuttallochiton mirandus ; satellite DNA
• 刊名：Chromosome Research
• 出版年：2008
• 出版时间：September, 2008
• 年：2008
• 卷：16
• 期：6
• 页码：907-916
• 全文大小：314.0 KB

文摘

Two highly repeated DNAs, designated NmE1/NmE2 and NmE5, were identified by EcoRV digestion in the chiton Nuttallochiton mirandus (Mollusca: Polyplacophora). The comparison of the sequences obtained showed high similarity in 5′ and 3′ regions and the NmE5 sequence displayed an inserted sequence that might arise from a transposable element. Southern blotting analyses suggested a tandem organization of both satellite DNA families identified. Moreover, dot blot analyses, performed on several molluscan species, revealed a different degree of conservation of the repeated DNAs. Fluorescence in-situ hybridizations (FISH) on metaphase chromosomes showed that both satellite DNAs are located at centromeric regions.