Molecular characterization and expression analysis of major histocompatibility complex class IIA and IIB genes of Odontobutis potamophila

详细信息    查看全文

• 作者：Fenfei Liang ; Yayuan Wang ; Panfeng Tao ; Guosong Zhang ; Shaowu Yin
• 刊名：Fisheries Science
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：82
• 期：4
• 页码：653-663
• 全文大小：1,152 KB
• 刊物主题：Fish & Wildlife Biology & Management; Freshwater & Marine Ecology; Food Science;
• 出版者：Springer Japan
• ISSN：1444-2906
• 卷排序：82

文摘

The major histocompatibility complex (MHC) has a critical role in the adaptive immune system by presenting a foreign peptide to the T cell receptor. The full length of MHC class IIA and IIB cDNA was cloned from Odontobutis potamophila by expressed sequence tag and rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). Tissue distribution and expression of MHCIIA and IIB were examined to study the functions of the MHCII gene in fish. The domain structure and antigen-binding motifs of other teleosts and mammals MHCII are conserved in O. potamophila as MHCIIA and IIB genes. The alignments of the deduced amino acid sequences from MHCIIA and MHCIIB in O. potamophila with those from other vertebrates demonstrated a 25–52 % similarity between other fish and mammalians. Real-time quantitative PCR demonstrated that MHCIIA and IIB genes were ubiquitously expressed in all tested tissues. The challenge of O. potamophila with Aeromonas hydrophila resulted in a significant change in the expression of MHCIIA and IIB mRNA in the liver, spleen and kidney. All these results suggest that both genes play crucial roles in immune responses of O. potamophila.KeywordsOdontobutis potamophilaMHCIIAMHCIIBcDNASequence analysisMRNA expression