1H, 13C and 15N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii
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- 作者：Himanshu Singh (1)
B. J. Rao (2)
Kandala V. R. Chary (1) (3) - 关键词：NMR ; Resonance assignments ; UVI31+ ; S55A ; Chlamydomonas reinhardtii
- 刊名：Biomolecular NMR Assignments
- 出版年：2014
- 出版时间：October 2014
- 年：2014
- 卷：8
- 期：2
- 页码：371-374
- 全文大小：786 KB
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B. J. Rao (2)
Kandala V. R. Chary (1) (3)
1. Department of Chemical Sciences, Tata Institute of Fundamental Research, 1, Homi Bhabha Road, Colaba, Mumbai, 400005, India
2. Department of Biological Sciences, Tata Institute of Fundamental Research, 1, Homi Bhabha Road, Mumbai, India
3. Center for Interdisciplinary Sciences, Tata Institute of Fundamental Research, 21, Brindavan Colony, Narsingi, Hyderabad, 500075, India - ISSN：1874-270X
文摘
Almost complete sequence specific 1H, 13C and 15N resonance assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii are reported, as a prelude to its structural and functional characterization. Site-directed mutagenesis of uvi31+ was carried out using complementary mutation harbouring oligonucleotides for S55A mutant. The resulting mutant was sequenced and then S55A mutant of uvi31+ cDNA were expressed in E. coli, and purification were carried out from where the protein was purified to high homogeneity. The point mutation S55A in UVI31+ results in a significant enhancement in its DNA endonuclease activity as compared to its wild type protein.