Linear B-cell epitope mapping of MAPK3 and MAPK4 from Leishmania braziliensis: implications for the serodiagnosis of human and canine leishmaniasis

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作者：Daniel Menezes-Souza (1) Tiago Ant么nio de Oliveira Mendes (1) Ana Carolina de Ara煤jo Le茫o (1) Matheus de Souza Gomes (2) Ricardo Toshio Fujiwara (1) Daniella Castanheira Bartholomeu (1)
关键词：Tegumentary and visceral human leishmaniasis ; Canine visceral leishmaniasis ; Immunodiagnosis ; MAPK3 ; MAPK4 ; B ; cell epitope mapping
刊名：Applied Microbiology and Biotechnology
出版年：2015
出版时间：February 2015
年：2015
卷：99
期：3
页码：1323-1336
全文大小：1,801 KB
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作者单位：Daniel Menezes-Souza (1) Tiago Ant么nio de Oliveira Mendes (1) Ana Carolina de Ara煤jo Le茫o (1) Matheus de Souza Gomes (2) Ricardo Toshio Fujiwara (1) Daniella Castanheira Bartholomeu (1) 1. Departamento de Parasitologia, Instituto de Ci锚ncias Biol贸gicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil 2. Instituto de Gen茅tica e Bioqu铆mica, Universidade Federal de Uberl芒ndia, Patos de Minas, Minas Gerais, Brazil
刊物类别：Chemistry and Materials Science
刊物主题：Chemistry Biotechnology Microbiology Microbial Genetics and Genomics
出版者：Springer Berlin / Heidelberg
ISSN：1432-0614

文摘

The correct and early identification of humans and dogs infected with Leishmania are key steps in the control of leishmaniasis. Additionally, a method with high sensitivity and specificity at low cost that allows the screening of a large number of samples would be extremely valuable. In this study, we analyzed the potential of mitogen-activated protein kinase 3 (MAPK3) and mitogen-activated protein kinase 4 (MAPK4) proteins from Leishmania braziliensis to serve as antigen candidates for the serodiagnosis of human visceral and tegumentary leishmaniasis, as well as canine visceral disease. Moreover, we mapped linear B-cell epitopes in these proteins and selected those epitopes with sequences that were divergent in the corresponding orthologs in Homo sapiens, in Canis familiaris, and in Trypanosoma cruzi. We compared the performance of these peptides with the recombinant protein using ELISA. Both MAPK3 and MAPK4 recombinant proteins showed better specificity in the immunodiagnosis of human and canine leishmaniasis than soluble parasite antigens and the EIE-leishmaniose-visceral-canina-bio-manguinhos (EIE-LVC) kit. Furthermore, the performance of this serodiagnosis assay was improved using synthetic peptides corresponding to B-cell epitopes derived from both proteins.

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