Exclusion of dysfunctional mitochondria from Balbiani body during early oogenesis of Thermobia

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• 作者：Waclaw Tworzydlo ; Elzbieta Kisiel ; Wladyslawa Jankowska…
• 关键词：Oogenesis ; Balbiani body ; Mitochondrial dynamics ; Mitochondrial network
• 刊名：Cell and Tissue Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：366
• 期：1
• 页码：191-201
• 全文大小：2,628 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878
• 卷排序：366

文摘

Oocytes of many invertebrate and vertebrate species contain a characteristic organelle complex known as the Balbiani body (Bb). Until now, three principal functions have been ascribed to this complex: delivery of germ cell determinants and localized RNAs to the vegetal cortex/posterior pole of the oocyte, transport of the mitochondria towards the germ plasm, and participation in the formation of lipid droplets. Here, we present the results of a computer-aided 3D reconstruction of the Bb in the growing oocytes of an insect, Thermobia domestica. Our analyses have shown that, in Thermobia, the central part of each fully developed Bb comprises a single intricate mitochondrial network. This “core” network is surrounded by several isolated bean-shaped mitochondrial units that display lowered membrane potential and clear signs of degeneration. In light of the above results and recent theoretical models of mitochondrial quality control, the role of the Bb is discussed. We suggest that, in addition to the aforementioned functions, the Bb is implicated in the selective elimination of dysfunctional mitochondria during oogenesis.