Molecular cloning, characterisation and expression of the translationally controlled tumor protein gene in rock bream (Oplegnathus fasciatus)

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• 作者：Yeong Sun Chae ; Jung-Ho Lee ; Won Joon Shim ; Jee-Hyun Jung ; Ju-Won Kim…
• 关键词：Rock bream ; Translationally controlled tumor protein ; Cloning ; Expression analysis
• 刊名：Genes & Genomics
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：36
• 期：5
• 页码：565-572
• 全文大小：1,835 KB
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• 作者单位：Yeong Sun Chae (1) (3)
  Jung-Ho Lee (2)
  Won Joon Shim (3)
  Jee-Hyun Jung (3)
  Ju-Won Kim (1)
  Chan-Il Park (1)
  
  1. Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam, 650-160, Republic of Korea
  3. Oil and POPs Research Group, Korea Institute of Ocean Science and Technology, 391 Jangbuk-Ri, Jangmok-Myon, Geoje, 656-834, Republic of Korea
  2. Genetics and Breeding Research Center, National Fisheries Research and Development Institute, Geoje, 656-842, Republic of Korea
  
• ISSN：2092-9293

文摘

Translationally controlled tumor protein (TCTP) is an important immune regulator that has been implicated in a number of cellular processes, including cell growth, cell cycle progression, apoptosis regulation and protection of cells against various environmental stresses. In this study, we cloned and characterised TCTP from rock bream (Oplegnathus fasciatus), which is an economically important species in the Korean aquaculture industry. The full-length rock bream TCTP (RbTCTP) cDNA was of 1,041?bp and contained an open reading frame of 513?bp, which encoded 170 amino acids. The 5-untranslated region (UTR) was 90?bp, while the 3-UTR was 438?bp, containing a polyadenylation signal. RbTCTP showed 76, 75 and 74?% amino acid sequence identities to those of tilapia (Oreochromis niloticus), orange-spotted grouper (Epinephelus coioides) and Japanese sea perch (Lateolabrax japonicus), respectively. The positions of microtubule binding region, Ca+ binding region and TCTP signature regions in RbTCTP were similar to other fish species and mammals. RbTCTP mRNA expression level was highest in the gill compared to other tissues. The level of RbTCTP mRNA expression was significantly regulated by injection of red seabream iridovirus, Streptococcus iniae and Edwardsiella tarda.