Molecular profile and expressional modulation of a Toll like receptor-1 homolog from rock bream (Oplegnathus fasciatus)

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• 作者：Don Anushka Sandaruwan Elvitigala ; H. K. A. Premachandra ; Sang-Yeob Yeo…
• 关键词：Rock bream ; TLR1 ; Genome organization ; Immune stimulants ; Transcriptional profiling
• 刊名：Genes & Genomics
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：37
• 期：5
• 页码：459-470
• 全文大小：3,137 KB
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• 作者单位：Don Anushka Sandaruwan Elvitigala (1) (2)
  H. K. A. Premachandra (1)
  Sang-Yeob Yeo (3)
  Cheol Young Choi (4)
  Ilson Whang (1) (2)
  Jehee Lee (1) (2)
  
  1. Marine Molecular Genetics Lab, Department of Marine Life Sciences, School of Marine Biomedical Sciences, College of Ocean Science, Jeju National University, 66 Jejudaehakno, Ara-Dong, Jeju Self-Governing Province, Jeju, 690-756, Republic of Korea
  2. Fish Vaccine Development Center, Jeju National University, Jeju Special Self-Governing Province, Jeju, 690-756, Republic of Korea
  3. Department of Biotechnology, Division of Applied Chemistry & Biotechnology, Hanbat National University, Daejeon, 305-719, Republic of Korea
  4. Division of Marine Environment & Bioscience, Korea Maritime University, Busan, 606-791, Republic of Korea
  
• 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
• 出版者：Springer Netherlands
• ISSN：2092-9293

文摘

Toll-like receptors (TLRs) are well-described pattern recognition receptors that recognize different pathogen-associated molecular patterns (PAMPs) to activate innate immune responses. TLR1 forms a heterodimer with TLR2 that works to identify PAMPs including bacterial components. In the present study, we identified a TLR1 homolog from rock bream Oplegnathus fasciatus (RbTLR1) and characterized it at the genomic level. The complete coding sequence of RbTLR1 was 2406?bp in length and encoded an 802 amino acid protein possessing a calculated molecular mass of 90.7?kDa and typical TLR domain architecture. A single exon spanned the genomic length (2931?bp) of RbTLR1. Phylogenetic analysis showed a close evolutionary relationship between RbTLR1 and its fish orthologs. Using quantitative real time polymerase chain reaction, we detected RbTLR1 mRNA in all the tissues examined, suggesting its merely universal tissue expression. RbTLR1 was expressed strongly in the spleen, kidney, and liver. Following immune stimulation by bacterial pathogens and mitogens, RbTLR1 transcript levels were elevated at various time points post stimulation. Moreover, injection of a viral stimulant, polyinosinic:polycytidylic acid, and rock bream iridovirus altered the basal transcription of RbTLR1. Collectively, these data showed that RbTLR1 is transcriptionally regulated in response to bacterial and viral stimulation, suggesting its putative importance in host immune defense.