Significant population genetic structure detected in the rock bream Oplegnathus fasciatus (Temminck & Schlegel, 1844) inferred from fluorescent-AFLP analysis
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- 作者：Yongshuang Xiao 肖永双 ; Daoyuan Ma 马道远…
- 刊名：Chinese Journal of Oceanology and Limnology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：34
- 期：3
- 页码：441-450
- 全文大小：470 KB
- 刊物主题：Oceanography;
- 出版者：Springer Berlin Heidelberg
- ISSN：1993-5005
- 卷排序：34
文摘
Oplegnathus fasciatus (rock bream) is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplified fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1 264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei’s genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance (AMOVA) showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a significant population genetic structure existed in the species. The pairwise fixation index Fst ranged from 0.20 to 0.63 and were significant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two significant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O. fasciatus populations examined should comprise two stocks.KeywordsOplegnathus fasciatusfluorescent-AFLPgenetic diversitygenetic structure