In vitro and in vivo activity of hyperimmune globulin preparations against multiresistant nosocomial pathogens

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• 作者：F. S. Rossmann ; A. Kropec ; D. Laverde ; F. R. Saaverda ; D. Wobser ; J. Huebner
• 关键词：Immune globuline preparations ; IgG ; IgM ; Nosocomial pathogens ; Opsonic killing ; Protective efficacy ; Animal model
• 刊名：Infection
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：43
• 期：2
• 页码：169-175
• 全文大小：298 KB
• 参考文献：1. Centers for Disease Control and Prevention CDC. Antibiotic resistance threats in the United States 2013 Internet www.cdc.gov 2013 cited 2014 Jul 3. pp. 1-14. Available from: http://www.cdc.gov/drugresistance/threat-report-2013/.
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Infectious Diseases
  General Practice and Family Medicine
  Internal Medicine
  
• 出版者：Urban & Vogel
• ISSN：1439-0973

文摘

Purpose We compared different immunoglobulin preparations containing IgG (Intraglobin/Intratect) or a mixture of IgG, IgA, and IgM (Pentaglobin) to assess the opsonic and protective efficacy of human immunoglobulin preparations against multiresistent nosocomial pathogens. Materials and methods Clinical isolates of E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis, Enterococcus faecium, and Staphylococcus aureus were tested by opsonophagocytic assay using immunologobulin preparations at dilutions usually obtained in patients. The target antigens of opsonic antibodies were characterized by opsonophagocytic inhibition assays, and the protective efficacy in vivo was tested in a mouse bacteremia model as previously described. Results All strains were killed to at least 50?% by Pentaglobin. One P. aeruginosa strain was not efficiently killed by Intraglobin (23?%)?but the other strains were killed by Intraglobin?to a similar degree compared to Pentaglobin. Opsonic IgG antibodies against E. faecalis were directed against LTA, while opsonic antibodies in Pentaglobin were primarily directed against other cell wall carbohydrates. In a mouse bacteremia model, Pentaglobin was more protective than Intratect?against Staphylococcus aureus, while Intratect reduced colony counts better than normal rabbit serum or saline. Conclusions All tested human immunoglobulin preparations contain opsonic and protective antibodies against targets present on multiresistant Gram-positive and Gram-negative bacteria. Enrichment of these preparations with IgM increases the protective efficacy against some strains, probably due to antibodies directed against cell wall carbohydrates.