Optical diagnosis of gallbladder cancers via two-photon excited fluorescence imaging of unstained histological sections
详细信息 查看全文
- 作者：Zhipeng Hong (1) (2)
Youting Chen (2)
Jing Chen (1)
Hong Chen (3)
Yahao Xu (1)
Xiaoqin Zhu (1) (4)
Shuangmu Zhuo (1)
Zheng Shi (2)
Jianxin Chen (1)
1. Institute of Laser and Optoelectronics Technology ; Fujian Provincial Key Laboratory for Photonics Technology ; Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education ; Fujian Normal University ; Fuzhou ; 350007 ; People鈥檚 Republic of China
2. Department of Hepatopancreatobiliary Surgery and Institute of Abdominal Surgery ; The First Affiliated Hospital of Fujian Medical University ; Fuzhou ; 350005 ; People鈥檚 Republic of China
3. Department of Pathology ; The First Affiliated Hospital of Fujian Medical University ; Fuzhou ; 350005 ; People鈥檚 Republic of China
4. Beckman Laser Institute and Medical Clinic ; University of California ; Irvine ; California 92612 ; USA - 关键词：Gallbladder cancers ; Diagnosis ; Unstained histological sections ; Two ; photon excited fluorescence imaging ; Pathology
- 刊名：Lasers in Medical Science
- 出版年：2015
- 出版时间：January 2015
- 年：2015
- 卷：30
- 期：1
- 页码：225-233
- 全文大小：17,760 KB
- 参考文献：1. Misra S, Chaturvedi A, Misra NC, Sharma ID (2003) Carcinoma of the gallbladder. Lancet Oncol 4:167鈥?76. doi:10.1016/S1470-2045(03)01021-0 CrossRef
2. Lai CH, Lau WY (2008) Gallbladder cancer鈥攁 comprehensive review. Surgeon 6:101鈥?10. doi:10.1016/S1479-666X(08)80073-X CrossRef
3. Laitio M (1983) Histogenesis of epithelial neoplasms of human gallbladder II: classification of carcinoma on the basis of morphological features. Pathol Res Pract 178:57鈥?6. doi:10.1016/S0344-0338(83)80086-7 CrossRef
4. Susumu N, Masami Y, Eiichi T (1989) Morphometric analysis of gallbladder adenocarcinoma: discrimination between carcinoma and dysplasia. Virchows Archiv A Pathol Anat 416:133鈥?40. doi:10.1007/BF01606318 CrossRef
5. Henson DE, Albores-Saavedra J, Corle D (1992) Carcinoma of the gallbladder: histologic types, stage of disease, grade, and survival rates. Cancer 70:1493鈥?497. doi:10.1002/1097-0142 CrossRef
6. Levy AD, Murakata LA, Rohrmann CA Jr (2001) Gallbladder carcinoma: radiologic-pathologic correlation. Radiographics 21:295鈥?14. doi:10.1148/radiographics.21.2.g01mr16295 CrossRef
7. Hundal R, Shaffer EA (2014) Gallbladder cancer: epidemiology and outcome. J Clin Epidemiol 6:99鈥?09. doi:10.2147/CLEP.S37357
8. Zevallos Maldonado C, Ruiz Lopez MJ, Gonzalez Valverde FM, Alarcon Soldevilla F, Pastor Quirante F, Garcia Medina V (2014) Ultrasound findings associated to gallbladder carcinoma. Cir Espen 92:348鈥?55. doi:10.1016/j.ciresp.2012.10.007 CrossRef
9. Ramos-Font C, Gomez-Rio M, Rodriguez-Fernandez A, Jimenez-Heffernan A, Sanchez Sanchez R, Llamas-Elvira JM (2014) Ability of FDG-PET/CT in the detection of gallbladder cancer. J Surg Oncol 109:218鈥?24. doi:10.1002/jso.23476 CrossRef
10. Lee NK, Kim S, Kim TU, Kim DU, Seo HI, Jeon TY (2014) Diffusion-weighted MRI for differentiation of benign from malignant lesions in the gallbladder. Clin Radiol 69:e78鈥揺85. doi:10.1016/j.crad.2013.09.017 CrossRef
11. Larson K, Ho HH, Anumolu PL, Chen TM (2011) Hematoxylin and eosin tissue stain in Mohs micrographic surgery: a review. Dermatol Surg 37:1089鈥?099. doi:10.1111/j.1524-4725.2011.02051.x CrossRef
12. Gill GW (2010) H&E Staining. In: Kumar GL, Kiernan JA (eds) Education guide: special stains and H&E, 2nd edn. Dako North America, California, pp 177鈥?84
13. Denk W, Strickler J, Webb WW (1990) Two-photon laser scanning fluorescence microscopy. Science 248:73鈥?6. doi:10.1126/science.2321027 CrossRef
14. Zipfel WR, Williams RM, Webb WW (2003) Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol 21:1369鈥?377. doi:10.1038/nbt899 CrossRef
15. Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT, Webb WW (2003) Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci USA 100:7075鈥?080. doi:10.1073/pnas.0832308100 CrossRef
16. Yan J, Chen G, Chen J, Liu N, Zhuo S, Yu H, Ying M (2011) A pilot study of using multiphoton microscopy to diagnose gastric cancer. Surg Endosc 25:1425鈥?430. doi:10.1007/s00464-010-1409-z CrossRef
17. Pavlova I, Hume KR, Yazinski SA, Peters RM, Weiss RS, Webb WW (2010) Multiphoton microscopy as a diagnostic imaging modality for lung cancer. Proc Soc Photo Opt Instrum Eng 7569:756918. doi:10.1117/12.841017
18. Wu X, Chen G, Lu J, Zhu W, Qiu J, Chen J, Xie S, Zhuo S, Yan J (2013) Label-free detection of breast masses using multiphoton microscopy. PLoS ONE 8:e65933. doi:10.1371/journal.pone.0065933 CrossRef
19. Madden KS, Zettel ML, Majewska AK, Brown EB (2013) Brain tumor imaging: live imaging of glioma by two-photon microscopy. Cold Spring Harb Protoc. doi:10.1101/pdb.prot073668 , 3: pdb-prot073668
20. Yan J, Zhuo S, Chen G, Wu X, Zhou D, Xie S, Jiang J, Ying M, Jia F, Chen J, Zhou J (2012) Preclinical study of using multiphoton microscopy to diagnose liver cancer and differentiate benign and malignant liver lesions. J Biomed Opt 17:0260041鈥?260047. doi:10.1117/1.JBO.17.2.026004 CrossRef
21. Tuer A, Tokarz D, Prent N, Cisek R, Alami J, Dumont DJ, Bakueva L, Rowlands J, Barzda V (2010) Nonlinear multicontrast microscopy of hematoxylin-and-eosin-stained histological sections. J Biomed Opt 15:026018. doi:10.1117/1.3382908 CrossRef
22. Chen J, Xu J, Kang D, Xu M, Zhuo S, Zhu X, Jiang X (2013) Multiphoton microscopic imaging of histological sections without hematoxylin and eosin staining differentiates carcinoma in situ lesion from normal oesophagus. Appl Phys Lett 103:183701. doi:10.1063/1.4826322 CrossRef
23. Xu J, Kang D, Xu M, Zhuo S, Zhu X, Chen J (2013) Multiphoton microscopic imaging of esophagus during the early phase of tumor progression. Scanning 35:387鈥?91. doi:10.1002/sca.21079 CrossRef
24. Manuelidis L (1984) Active nucleolus organizers are precisely positioned in adult central nervous system cells but not in neuroectodermal tumor cells. J Neuropathol Exp Neurol 43:225鈥?41 CrossRef
25. R眉schoff J (1992) Nucleolus organizer regions in pathomorphologic tumor diagnosis. Veroff Pathol 139:1鈥?44 - 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Medicine/Public Health, general
Dentistry
Laser Technology and Physics and Photonics
Quantum Optics, Quantum Electronics and Nonlinear Optics
Applied Optics, Optoelectronics and Optical Devices - 出版者：Springer London
- ISSN：1435-604X
文摘
Two-photon excited fluorescence (TPEF) microscopy, based on signal from cells, can provide detailed information on tissue architecture and cellular morphology in unstained histological sections to generate subcellular-resolution images from tissue directly. In this paper, we used TPEF microscopy to image microstructure of human normal gallbladder and three types of differentiated carcinomas in order to investigate the morphological changes of tissue structure, cell, cytoplasm, and nucleus without hematoxylin and eosin (H&E) staining. It displayed that TPEF microscopy can well image the stratified normal gallbladder tissue, including the mucosa, the muscularis, and the serosa. The typical cancer cell, characterized by cellular and nuclear pleomorphism, enlarged nuclei, and augmented nucleolus, can be identified in histological sections without H-E staining as well. The quantitative results showed that the areas of the nucleus and the nucleolus in three types of cancerous cells were all significantly greater than those in normal gallbladder columnar epithelial cells derived from TPEF microscopic images. The studies demonstrated that TPEF microscopy has the ability to characterize tissue structures and cell morphology of gallbladder cancers differentiated from a normal gallbladder in a manner similar to traditional histological analysis. As a novel tool, it has the potential for future retrospective studies of tumor staging and migration by utilizing histological section specimens without H-E staining.