Microbial community analysis of a full-scale DEMON bioreactor

详细信息    查看全文

• 作者：Alejandro Gonzalez-Martinez (1)
  Alejandro Rodriguez-Sanchez (2)
  Barbara Mu帽oz-Palazon (2)
  Maria-Jesus Garcia-Ruiz (1)
  Francisco Osorio (1)
  Mark C. M. van Loosdrecht (3)
  Jesus Gonzalez鈥揕opez (2)
  
  1. Department of Civil Engineering ; University of Granada ; Campus de Fuentenueva ; s/n ; 18071 ; Granada ; Spain
  2. Institute of Water Research ; University of Granada ; C/Ram贸n y Cajal ; 4 ; 18071 ; Granada ; Spain
  3. Department of Biotechnology ; Technical University of Delft ; Julianalaan 67 ; 2628 BC ; Delft ; The Netherlands
  
• 关键词：Anammox ; Deammonification ; DEMON ; Pyrosequencing ; Wastewater treatment
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：38
• 期：3
• 页码：499-508
• 全文大小：2,237 KB
• 参考文献：1. Rodriguez-Sanchez, A, Gonzalez-Martinez, A, Martinez-Toledo, MV, Garcia-Ruiz, MJ, Osorio, F, Gonzalez-Lopez, J (2014) The effect of influent characteristics and operational conditions over the performance and microbial community structure of partial nitritation reactors. Water 6: pp. 1905-1924 CrossRef
  2. Kotay, SM, Mansell, BL, Hogsett, M, Pei, H, Goel, R (2013) Anaerobic ammonia oxidation (ANAMMOX) for side-stream treatment of anaerobic digester filtrate process performance and microbiology. Biotechnol Bioeng 110: pp. 1180-1192 CrossRef
  3. Shen, LD, Liu, S, Lou, LP, Liu, WP, Xu, XY, Zheng, P, Hu, BI (2013) Broad distribution of diverse anaerobic ammonium-oxidizing bacteria in chinese agricultural soils. Appl Environ Microbiol 79: pp. 6167-6172 CrossRef
  4. Ganesh, S, Parris, DJ, DeLong, EF, Stewart, FJ (2014) Metagenomic analysis of size-fractionated picoplankton in a marine oxygen minimum zone. ISMEJ 8: pp. 187-211 CrossRef
  5. Thamdrup, B, Jensen, MM, Ulloa, O, Faria, L, Escribano, R (2006) Anaerobic ammonium oxidation in the oxygen-deficient waters off northern Chile. Limnol Oceanogr 51: pp. 2145-2156 CrossRef
  6. Innerebner, G, Insam, H, Franke-Whittle, IH, Wett, B (2007) Identification of anammox bacteria in a full-scale deammonification plant making use of anaerobic ammonia oxidation. Syst Appl Microbiol 30: pp. 408-412 CrossRef
  7. Gonzalez-Martinez, A, Rodriguez-Sanchez, A, Martinez-Toledo, MV, Garcia-Ruiz, MJ, Hontoria, E, Osorio-Robles, F, Gonzalez-Lopez, J (2014) Effect of ciprofloxacin antibiotic on the partial-nitritation process and bacterial community structure of a submerged biofilter. Sci Total Environ 476鈥?77: pp. 276-287 CrossRef
  8. Dalahmeh, SS, J枚nson, H, Hylander, LD, Hui, N, Yu, D, Pell, M (2014) Dynamics and functions of bacterial communities in bark, charcoal and sand filters treating greywater. Water Res 54: pp. 21-32 CrossRef
  9. Han, D, Kang, I, Ha, HK, Kim, HC, Kim, OS, Lee, BY, Cho, JC, Hur, HG, Lee, YH (2014) Bacterial communities of surface mixed layer in the Pacific sector of the Western Arctic Ocean during sea-ice melting. PLoS One 9: pp. e86887 CrossRef
  10. Huang, Q, Jiang, H, Briggs, BR, Wang, S, Hou, W, Li, G, Wu, G, Solis, R, Arcilla, CA, Abrajano, T, Dong, H (2013) Archaeal and bacterial diversity in acidic circumneutral hot springs in Philippines. FEMS Microbiol 85: pp. 452-464 CrossRef
  11. Livermore, JA, Jin, YO, Arnseth, RW, LePuil, M, Mattes, TE (2013) Microbial community dynamics during acetate biostimulation of RDX-contaminated groundwater. Environ Sci Technol 47: pp. 7672-7678 CrossRef
  12. Zekker, I, Rikmann, E, Tenno, T, Saluste, A, Tomingas, M, Menert, A, Loorits, L, Lemmiksoo, V, Tenno, T (2012) Achieving nitritation and anammox enrichment in a single moving-bed biofilm reactor treating reject water. Environ Technol 33: pp. 703-710 CrossRef
  13. Mozumder, MSI, Picioreanu, C, Loosdrecht, MCM, Volcke, EIP (2014) Effect of heterotrophic growth on autotrophic nitrogen removal in a granular sludge reactor. Environ Technol 35: pp. 1027-1037 CrossRef
  14. Sorokin, DY, L眉cker, S, Vejmelkova, D, Kostrikina, NA, Kleerebezem, R, Rijpstra, WIC, Damst茅, JSS, Paslier, D, Muyzer, G, Wagner, M, Loosdrecht, MCM, Daims, H (2012) Nitrification expanded: discovery, physiology and genomics of a nitrite-oxidizing bacterium from the phylum Chloroflexi. ISMEJ 6: pp. 2245-2256 CrossRef
  15. Dowd, SE, Sun, Y, Secor, PR, Rhoads, DD, Wolcott, BM, James, GA, Wolcott, RD (2008) Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol 8: pp. 43 CrossRef
  16. Nakayama, J (2010) Pyrosequence-based 16S rRNA profiling of gastro-intestinal microbiota. Biosci Microflora 29: pp. 83-96 CrossRef
  17. Sogin, ML, Morrison, HG, Huber, JA, Welch, DM, Huse, SM, Neal, PR, Arrieta, JM, Herndl, GJ (2006) Microbial diversity in the deep sea and the underexplored 鈥渞are biosphere鈥? PNAS 103: pp. 12115-12120 CrossRef
  18. Dowd, SE, Callaway, TR, Wolcott, RD, Sun, Y, McKeehan, T, Hagevoort, RG, Edrington, TS (2008) Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). BMC Microbiol 8: pp. 125 CrossRef
  R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna
  19. Park, H, Rosenthal, A, Ramalingam, K, Fillos, J, Chandran, K (2010) Linking community profiles, gene expression and N-removal in anammox bioreactors treating municipal anaerobic digestion reject water. Environ Sci Technol 44: pp. 6110-6116 CrossRef
  20. Kant, R, Passel, MWJ, Sangwan, P, Palva, A, Lucas, S, Copeland, A (2011) Genome sequence of 鈥淧edosphaera parvula鈥?Ellin514, an aerobic Verrucomicrobial isolate from pasture soil. J Bacteriol 193: pp. 2900-2901 CrossRef
  21. Sangwan, P, Kovac, S, Davis, KER, Sait, M, Janssen, PH (2005) Detection and Cultivation of Soil Verrucomicrobia. Appl Environ Microbiol 71: pp. 8402-8410 CrossRef
  22. Galperin, MY (2008) New feel for new phyla. Environ Microbiol 10: pp. 1927-1933 CrossRef
  23. Chouari, R, Paslier, D, Dauga, C, Daegelen, P, Weissenbach, J, Sghir, A (2005) Novel major bacteria candidate division within a municipal anaerobic sludge digester. Appl Environ Microbiol 71: pp. 2145-2153 CrossRef
  24. Haldar, S, Choudhury, SR, Sengupta, S (2011) Genetic and functional diversities of bacterial communities in the rhizosphere of Arachis hypogaea. Antonie Van Leeuwenhoek 100: pp. 161-170 CrossRef
  25. Shimizu, T, Ohtani, K, Hirakawa, H, Ohshima, K, Yamashita, A, Shiba, T (2002) Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. Proc Natl Acad Sci USA 99: pp. 996-1001 CrossRef
  26. Bruggemann, H, Baumer, S, Fricke, WF, Wiezer, A, Liesegang, H, Decker, I (2003) The genome sequence of Clostridium tetani, the causative agent of tetanus disease. Proc Natl Acad Sci USA 100: pp. 1316-1321 CrossRef
  27. N枚lling, J, Breton, G, Omelchenko, MV, Kira, S, Zeng, Q, Gibson, R (2001) Genome sequence and comparative analysis of the solvent-producing Bacterium Clostridium acetobutylicum. J Bacteriol 183: pp. 4823-4838 CrossRef
  28. Miura, Y, Watanabe, Y, Okabe, S (2007) Significance of Chloroflexi in performance of submerged membrane bioreactors (MBR) treating municipal wastewater. Environ Sci Technol 41: pp. 7787-7794 CrossRef
  29. Nelson, MC, Morrison, M, Yu, Z (2011) A meta-analysis of the microbial diversity observed in anaerobic digesters. Biores Technol 102: pp. 3730-3739 CrossRef
  30. Strous, M, Fuerst, JA, Kramer, EHM, Logemann, S (1999) Missing lithotroph identified as new planctomycete. Nature 400: pp. 446-449 CrossRef
  31. Yoon, JH, Kim, IG, Oh, TK, Park, YH (2004) Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. Int J Syst Evol Microbiol 54: pp. 1111-1116 CrossRef
  32. Miyazaki, M, Nogi, Y, Ohta, Y, Hatada, Y, Fujiwara, Y, Ito, S, Horikoshi, K (2008) Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment. Int J Syst Evol Microbiol 58: pp. 1128-1133 CrossRef
  33. Gonzalez, JM, Mayer, F, Moran, MA, Hodson, RE, Whitman, WB (1997) Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. Int J Syst Bacteriol 47: pp. 369-376 CrossRef
  34. Guo, F, Zhang, S-H, Yu, X, Wei, B (2011) Variations of both bacterial community and extracellular polymers: the inducements of increase of cell hydrophobicity from biofloc to aerobic granule sludge. Biores Technol 102: pp. 6421-6428 CrossRef
  35. Gonzalez-Martinez, A, Calderon, K, Albuquerque, A, Hontorio, E, Gonzalez-Lopez, J, Guisado, IM, Osorio, F (2013) Biological and technical study of a partial-SHARON reactor at laboratory scale: effect of hydraulic retention time. Bioprocess Biosyst Eng 36: pp. 173-184 CrossRef
  36. Yoo, SH, Weon, HY, Jang, HB, Kim, BY, Kwon, SW, Go, SJ, Stackebrandt, E (2007) Sphingobacterium composti sp. nov., isolated from cotton-waste composts. Int J Syst Evol Microbiol 57: pp. 1590-1593 CrossRef
  37. Bao, HX, Ma, XP, Wang, J, Jing, K, Shen, ML, Wang, AJ (2012) Performance of denitrifying phosphorous removal process employing nitrite as electron acceptor and the characterization of dominant functional populations. Adv Mater Res 455鈥?56: pp. 1019-1024
  38. Nagashima, S, Kamimura, A, Shimizu, T, Nakamura-Isaki, S, Aono, E, Sakamoto, K (2012) Complete genome sequence of phototrophic betaproteobacterium Rubrivivax gelatinosus IL144. J Bacteriol 194: pp. 3541-3542 CrossRef
  39. Xie, B, Lv, Z, Hu, C, Yang, X, Li, X (2013) Nitrogen removal through different pathways in an aged refuse bioreactor treating mature landfill leachate. Appl Microbiol Biotechnol 97: pp. 9225-9234 CrossRef
  40. Lam, P, Jensen, MM, Lavik, G, McGinnis, DF, M眉ller, B, Schubert, CJ, Amann, R, Thamdrup, B, Kuypers, MMM (2007) Linking crenarchaeal and bacterial nitrification to anammox in the Black Sea. Proc Natl Acad Sci USA 104: pp. 7104-7109 CrossRef
  41. Fahrbach, M, Kuever, J, Remesch, M, Huber, BE, K盲mpfer, P, Dott, W, Hollender, J (2008) Steroidobacter denitrificans gen. nov., sp. nov., a steroidal hormone-degrading gammaproteobacterium. Int J Syst Evol Microbiol 58: pp. 2215-2223 CrossRef
  42. Glavina Del Rio, T, Abt, B, Spring, S, Lapidus, A, Nolan, M, Tice, H (2010) Complete genome sequence of Chitinophaga pinensis type strain (UQM 2034). Stand Gen Sci 2: pp. 87-95 CrossRef
  43. Chen, HJ, Lin, YZ, Fanjiang, JM, Fan, C (2013) Microbial community and treatment ability investigation in AOAO process for the optoelectronic wastewater treatment using PCR-DGGE biotechnology. Biodegradation 24: pp. 227-243 CrossRef
  44. Suen, G, Weimer, PJ, Stevenson, DM, Aylward, FO, Boyum, J, Deneke, J (2011) The complete genome sequence of Fibrobacter succinogenes S85 reveals a cellulolytic and metabolic specialist. PloS One 6: pp. e18814 CrossRef
  45. Woyke, T, Chertkov, O, Lapidus, A, Nolan, M, Lucas, S, Rio, TG (2011) Complete genome sequence of the gliding freshwater bacterium Fluviicola taffensis type strain (RW262). Stand Gen Sci 5: pp. 21-29 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

Full-scale applications of autotrophic nitrogen removal technologies for the treatment of digested sludge liquor have proliferated during the last decade. Among these technologies, the aerobic/anoxic deammonification process (DEMON) is one of the major applied processes. This technology achieves nitrogen removal from wastewater through anammox metabolism inside a single bioreactor due to alternating cycles of aeration. To date, microbial community composition of full-scale DEMON bioreactors have never been reported. In this study, bacterial community structure of a full-scale DEMON bioreactor located at the Apeldoorn wastewater treatment plant was analyzed using pyrosequencing. This technique provided a higher-resolution study of the bacterial assemblage of the system compared to other techniques used in lab-scale DEMON bioreactors. Results showed that the DEMON bioreactor was a complex ecosystem where ammonium oxidizing bacteria, anammox bacteria and many other bacterial phylotypes coexist. The potential ecological role of all phylotypes found was discussed. Thus, metagenomic analysis through pyrosequencing offered new perspectives over the functioning of the DEMON bioreactor by exhaustive identification of microorganisms, which play a key role in the performance of bioreactors. In this way, pyrosequencing has been proven as a helpful tool for the in-depth investigation of the functioning of bioreactors at microbiological scale.