Aluminum Trichloride Inhibits the Rat Osteoblasts Mineralization In Vitro
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- 作者：Miao Song ; Hui Huo ; Zheng Cao ; Yanfei Han ; Li Gao
- 关键词：Aluminum trichloride ; Mineralization ; Osteoblasts ; Rat
- 刊名：Biological Trace Element Research
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：175
- 期：1
- 页码：186-193
- 全文大小：
- 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
- 出版者：Springer US
- ISSN：1559-0720
- 卷排序：175
文摘
Aluminum (Al) is an accumulative toxic metal. Excessive Al accumulation inhibits osteoblasts mineralization and induces osteoporosis. However, the inhibition mechanism of Al on the mineralization is not fully understood. Thus, in this study, the rat osteoblasts were cultured and exposed to 0 mmol L−1 (control group, CG) and 0.52 mmol L−1 aluminum trichloride (AlCl3, treatment group, TG) for 7, 14, and 21 days, respectively. We found that mineralized matrix nodules, the activity of bone alkaline phosphatase, the concentration of extracellular calcium, the mRNA expression of type-I collagen, the mRNA and protein expressions of osteopontin, osteocalcin, and bone sialoprotein were all decreased, while the concentration of extracellular phosphorus was increased in TG compared with CG with time prolonged. Taken together, these results indicated that AlCl3 inhibited osteoblasts mineralization in vitro.