High-affinity ouabain receptors: primary membrane sensors for ionizing radiation

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• 作者：A. I. Dvoretsky (1)
  A. M. Shainskaya (2)
  S. N. Ayrapetyan (3) info@biophys.am
• 关键词：γ ; radiation &#8211 ; Neuron &#8211 ; Na/K pump &#8211 ; Na+/Ca2+ exchange &#8211 ; Isoform &#8211 ; Receptor
• 刊名：The Environmentalist
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：32
• 期：2
• 页码：242-248
• 全文大小：423.1 KB
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• 作者单位：1. Oles Honchar Dnepropetrovsk National University, Dnepropetrovsk, Ukraine2. Weizmann Institute in Rehovot, Rehovot, Israel3. UNESCO Chair-Life Sciences International Postgraduate Educational Center, Yerevan, Armenia
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Nature Conservation
  Ecology
  Economic Geology
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-2991

文摘

Although the high sensitivity of the Na/K pump in cell membrane to ionizing radiation is well known in literature, the individual role of different isoforms of pump in determination of its radio-sensitivity is not clear yet. This is the subject of the present investigation. Using isotope, electro-physiological and enzymological methods, the effect of γ-ionizing radiation on cell membrane voltage-current characteristics, acetylcholine-induced membrane current, 22Na+ and 45Ca2+ exchange between cells and bathing solution, Na+K+-ATPase activity, dose-dependent ouabain binding with cell membrane, intracellular cAMP and membrane phosphorylation in snail neurons were studied. The changes in neurons as a result of 30-min γ-radiation exposure of snails to 5.16 Ci/kg at the end of the first 30 min of post-radiation period were as follows: the increase in membrane ionic conductance reversed the ouabain sensitivity of acetylcholine-induced currents, stimulation of 22Na+ and 45Ca2+ uptakes, inhibition of Na/K pump, activation Na/Ca exchange in reversed mode, increase in ouabain binding with high-affinity α₃ and decrease with α₂ middle-affinity receptors, decrease in intracellular cAMP content and membrane dephosphorylation. On the basis of the obtained data, it is suggested that both α₃ and α₂ catalytic subunits of Na++K+-ATPase serve primary membrane sensors through the activation of which the biological effect of γ-radiation on neurons is realized. The IR has activation effects on α₃-dependent Na+/Ca2+ exchange in forward and its inactivation on α₂-dependent reverse modes.