Uncultured bone marrow mononuclear cells delay the dedifferentiation of unexpanded chondrocytes in pellet culture

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• 作者：Xiao Ouyang ; Bo Wei ; Fengyong Mao ; Xiang Zhang ; Yan Xu…
• 关键词：Bone marrow mononuclear cells ; Chondrocytes ; Dedifferentiation ; Cartilage tissue engineering ; Pellet culture
• 刊名：Cell and Tissue Research
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：361
• 期：3
• 页码：811-821
• 全文大小：5,861 KB
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  Mithoefer K, Williams RJ 3rd, Warren RF, Potter H
• 作者单位：Xiao Ouyang (1) (2) (3) (4)
  Bo Wei (1) (2) (3)
  Fengyong Mao (1) (2)
  Xiang Zhang (1) (2)
  Yan Xu (1) (2) (3)
  Liming Wang (1) (2) (3)
  
  1. Department of Orthopedics, Nanjing First Hospital, Nanjing Medical University, PO Box 210006, Nanjing, Jiangsu, China
  2. Cartilage Regeneration Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
  3. China-Korea United Cell Therapy Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, People’s Republic of China
  4. Department of Orthopedics, Xuzhou 3th Hospital, Affiliated Hospital of Jiangsu University, Xuzhou, Jiangsu, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

Uncultured bone marrow mononuclear cells (BMMC) were recently used to successfully repair damaged cartilage. However, the effect of BMMCs on the proliferation and differentiation of chondrocytes that are critical to cartilage repair is unclear. Here, we investigate the influence of BMMCs on chondrocyte dedifferentiation in pellet culture. We isolated and mixed BMMCs and chondrocytes in a 1:1 (BMMC/C) ratio and cultured in pellets (1.6?×-06 cells per pellet) for 2, 4, or 8 weeks. Chondrocyte differentiation was evaluated using macrography, histological examination, immunohistochemistry and gene expression analysis. While a transparent and smooth surface was observed in both BMMC/C and chondrocyte cultures over time, the former was smaller in size after 2 and 4 weeks of culture. Interestingly, after 8 weeks, BMMC/C cultures became significantly larger than chondrocyte cultures (P--.003). The distribution of a cartilage-specific extracellular matrix (ECM), that includes components like glycosaminoglycan (GAG) and type II collagen, was gradually reduced in chondrocyte cultures. On the other hand, while we found no obvious differences in the ECM in BMMC/C cultures between 2 and 4 weeks in vitro, after 8 weeks the concentration of ECM components decreased significantly. Further, we detected an upregulation of cartilage-specific genes in BMMC/C cultures, when compared with chondrocytes. Altogether, we demonstrate that co-culture with BMMCs delays the dedifferentiation of chondrocytes in pellet cultures in vitro. This suggests that uncultured BMMC, which can be quickly and safely obtained, could serve as a potential alternative cell source for engineering of cartilage tissue. Keywords Bone marrow mononuclear cells Chondrocytes Dedifferentiation Cartilage tissue engineering Pellet culture