The expression of annexin II and Lewis y antigen in ovarian epithelial tumors and the correlation between them

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• 作者：Huiyu Zhuang ; Mingzi Tan ; Juanjuan Liu ; Xiao Li ; Jian Gao ; Zhenhua Hu…
• 关键词：Ovarian epithelial carcinoma ; Annexin II ; Lewis y antigen ; Co ; immunoprecipitation ; Immunohistochemistry
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：36
• 期：4
• 页码：2343-2349
• 全文大小：491 KB
• 参考文献：1.Moriwaki K, Miyoshi E. Fucosylation and gastrointestinal cancer. World J Hepatol. 2010;2:151鈥?1.View Article PubMed Central PubMed
  2.Chhieng DC, Rodriguez-Burford C, Talley LI, Sviglin H, Stockard CR, Kleinberg MJ, et al. Expression of cea, tag-72, and lewis-y antigen in primary and metastatic lesions of ovarian carcinoma. Hum Pathol. 2003;34:1016鈥?1.View Article PubMed
  3.Li Q, Liu S, Lin B, Yan L, Wang Y, Wang C, et al. Expression and correlation of lewis y antigen and integrins alpha5 and beta1 in ovarian serous and mucinous carcinoma. Int J Gynecol Cancer : Off J Int Gynecol Cancer Soc. 2010;20:1482鈥?.
  4.Yin BW, Finstad CL, Kitamura K, Federici MG, Welshinger M, Kudryashov V, et al. Serological and immunochemical analysis of lewis y (ley) blood group antigen expression in epithelial ovarian cancer. Int J Cancer J Int du Cancer. 1996;65:406鈥?2.View Article
  5.Liu JJ, Lin B, Hao YY, Li FF, Liu DW, Qi Y, et al. Lewis(y) antigen stimulates the growth of ovarian cancer cells via regulation of the epidermal growth factor receptor pathway. Oncol Rep. 2010;23:833鈥?1.View Article PubMed
  6.Yan LM, Lin B, Zhu LC, Hao YY, Qi Y, Wang CZ, et al. Enhancement of the adhesive and spreading potentials of ovarian carcinoma rmg-1 cells due to increased expression of integrin alpha5beta1 with the lewis y-structure on transfection of the alpha1,2-fucosyltransferase gene. Biochimie. 2010;92:852鈥?.View Article PubMed
  7.Gao L, Yan L, Lin B, Gao J, Liang X, Wang Y, et al. Enhancive effects of lewis y antigen on cd44-mediated adhesion and spreading of human ovarian cancer cell line rmg-i. J exp Clin Cancer Res: CR. 2011;30:15.View Article PubMed Central PubMed
  8.Wang X, Gu J, Ihara H, Miyoshi E, Honke K, Taniguchi N. Core fucosylation regulates epidermal growth factor receptor-mediated intracellular signaling. J Biol Chem. 2006;281:2572鈥?.View Article PubMed
  9.Orczyk-Pawilowicz M. [The role of fucosylation of glycoconjugates in health and disease]. Postepy higieny i medycyny doswiadczalnej (Online). 2007;61:240鈥?2.
  10.Sharma M, Ownbey RT, Sharma MC. Breast cancer cell surface annexin ii induces cell migration and neoangiogenesis via tpa dependent plasmin generation. Exp Mol Pathol. 2010;88:278鈥?6.View Article PubMed
  11.Dai Z, Liu YK, Cui JF, Shen HL, Chen J, Sun RX, et al. Identification and analysis of altered alpha1,6-fucosylated glycoproteins associated with hepatocellular carcinoma metastasis. Proteomics. 2006;6:5857鈥?7.View Article PubMed
  12.Gu J, Taniguchi N. Potential of n-glycan in cell adhesion and migration as either a positive or negative regulator. Cell Adhes Migr. 2008;2:243鈥?.View Article
  13.Madjd Z, Parsons T, Watson NF, Spendlove I, Ellis I, Durrant LG. High expression of lewis y/b antigens is associated with decreased survival in lymph node negative breast carcinomas. Breast Cancer Res: BCR. 2005;7:R780鈥?87.View Article PubMed Central PubMed
  14.Arai Y, Nishida M. Differential diagnosis between normal endometrium and endometrial hyperplasia with immunostaining cytology using anti-ley monoclonal antibody. Int J Gynecol Cancer : Off J Int Gynecol Cancer Soc. 2003;13:42鈥?.View Article
  15.Kim YS, Yuan M, Itzkowitz SH, Sun QB, Kaizu T, Palekar A, et al. Expression of ley and extended ley blood group-related antigens in human malignant, premalignant, and nonmalignant colonic tissues. Cancer Res. 1986;46:5985鈥?2.PubMed
  16.Benaud C, Gentil BJ, Assard N, Court M, Garin J, Delphin C, et al. Ahnak interaction with the annexin 2/s100a10 complex regulates cell membrane cytoarchitecture. J Cell Biol. 2004;164:133鈥?4.View Article PubMed Central PubMed
  17.Laohavisit A, Davies JM. Annexins. New Phytologist. 2011;189:40鈥?3.View Article PubMed
  18.Bandorowicz-Pikula J, Wos M. Pikula S: [participation of annexins in signal transduction, regulation of plasma membrane structure and membrane repair mechanisms]. Postepy Biochem. 2012;58:135鈥?8.PubMed
  19.Zhang HJ, Yao DF, Yao M, Huang H, Wu W, Yan MJ, et al. Expression characteristics and diagnostic value of annexin a2 in hepatocellular carcinoma. World J Gastroenterol: WJG. 2012;18:5897鈥?04.View Article PubMed Central PubMed
  20.Shetty PK, Thamake SI, Biswas S, Johansson SL, Vishwanatha JK. Reciprocal regulation of annexin a2 and egfr with her-2 in her-2 negative and herceptin-resistant breast cancer. PLoS One. 2012;7:e44299.View Article PubMed Central PubMed
  21.Meng Q, Lei T, Zhang M, Zhao J, Zhao XH, Zhang M. Identification of proteins differentially expressed in adriamycin-resistant (pumc-91/adm) and parental (pumc-91) human bladder cancer cell lines by proteome analysis. J Cancer Res Clin Oncol. 2013;139:509鈥?9.View Article PubMed
  22.Seckinger A, Meissner T, Moreaux J, Depeweg D, Hillengass J, Hose K, et al. Clinical and prognostic role of annexin a2 in multiple myeloma. Blood. 2012;120:1087鈥?4.View Article PubMed
  23.Liu A, Liu D, Zhao S, Zheng J, Cao D, Zhang H. Up regulation of annexin a2 on murine h22 hepatocarcinoma cells induced by cartilage polysaccharide. Cancer Epidemiol. 2011;35:490鈥?.View Article PubMed
  24.Zhuang H, Gao J, Hu Z, Liu J, Liu D, Lin B. Co-expression of lewis y antigen with human epididymis protein 4 in ovarian epithelial carcinoma. PLoS One. 2013;8:e68994.View Article PubMed Central PubMed
  25.Kirchhoff C. Molecular characterization of epididymal proteins. Rev Reprod. 1998;3:86鈥?5.View Article PubMed
  
• 作者单位：Huiyu Zhuang (1)
  Mingzi Tan (1)
  Juanjuan Liu (1)
  Xiao Li (1)
  Jian Gao (1)
  Zhenhua Hu (1)
  Lu Deng (1)
  Liancheng Zhu (1)
  Bei Lin (1) (2)
  
  1. Department of Obstetrics and Gynecology, Shengjing Hospital affiliated to China Medical University, Shenyang, 110004, Liaoning, China
  2. Department of Obstetrics and Gynecology, China Medical University Shengjing Hospital, No. 36 Sanhao Street, Heping District, Shenyang, Liaoning, 110004, People鈥檚 Republic of China
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

The main aim of this study was to explore the molecular structural relationship between annexin II (ANXA2) and Lewis y antigen by determining their expression patterns and clinical significance in ovarian epithelial carcinoma. The structural relationship between ANXA2 and Lewis y antigen was examined using immunoprecipitation and confocal laser scanning microscopy in two ovarian caner cell lines ES-2 and CaoV-3. We also constracted the stably transfected cell lines with low ANXA2 gene expression in order to detect the expression level between ANXA2 and Lewis y. ANXA2 and Lewis y were detected in tissues from malignant, borderline, benign, and normal ovarian tissues using immunohistochemical analysis. ANXA2 and Lewis y were present in both two ovarian cancer cells and ANXA2 contained Lewis y antigen. Moreover, expression of Lewis y antigen in ANXA2 from cell after transfection was higher than that before. Our immunohistochemistry data revealed significantly higher positive expression rates of ANXA2 in malignant ovarian tissues, compared to benign tumor and normal tissue, similar to Lewis y antigen levels in ovarian cancer. Notably, tissues displaying marked expression of ANXA2 simultaneously expressed high levels of Lewis y antigen. A linear correlation between the expression patterns of ANXA2 and Lewis y antigen was evident. Consistently, double-labeling immunofluorescence experiments illustrated co-localization of ANXA2 and Lewis y antigen within the same area. In conclusions, ANXA2 contains Lewis y antigen. Our results further demonstrate a close correlation between the expression levels of the two antigens, which are significantly high in ovarian cancer.