Novel cyclohexane monooxygenase from Acidovorax sp. CHX100

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• 作者：Diego Salamanca ; Rohan Karande ; Andreas Schmid…
• 关键词：Cyclohexane ; CYP450 monooxygenase ; Cyclohexanol ; Cycloalkanes ; Biodegradation
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：99
• 期：16
• 页码：6889-6897
• 全文大小：449 KB
• 参考文献：Alexander A (2010) Bioremediation and biocatalysis with Polaromonas strain JS666. PhD thesis, University of Iowa
  Anderson MS, Hall R, Griffin M (1980) Microbial metabolism of alicyclic hydrocarbons: cyclohexane catabolism by a pure strain of Pseudomonas sp. J Gen Microbiol 120:89-4. doi:10.-099/-0221287-120-1-89
  Blanchette BN, Singh BR (2002) Induction of glutathione-S-transferase in the Northern quahog Mercenaria mercenaria after exposure to the polychlorinated biphenyl(PCB) mixture Aroclor 1248. J Protein Chem 21:489-94. doi:10.-023/?A:-022872619925 PubMed View Article
  Brzostowicz PC, Walters DM, Jackson RE, Halsey KH, Ni H, Rouviere PE (2005) Proposed involvement of a soluble methane monooxygenase homologue in the cyclohexane-dependent growth of a new Brachymonas species. Environ Microbiol 7:179-90. doi:10.-111/?j.-462-2920.-004.-0681.?x PubMed View Article
  Cheng Q, Thomas SM, Kostichka K, Valentine JR, Nagarajan V (2000) Genetic analysis of a gene cluster for cyclohexanol oxidation in Acinetobacter sp. strain SE19 by in vitro transposition. J Bacteriol 17:4744-751. doi:10.-128/?JB.-82.-7.-744-4751.-000 View Article
  Farinas ET, Schwaneberg U, Glieder A, Frances H (2001) Directed evolution of a cytochrome P450 monooxygenase for alkane oxidation. Adv Synth Catal 343:601-06. doi:10.-002/-615-4169(200108)343:- View Article
  Fortin PD, Horsman GP, Yang HM, Eltis LD (2006) A glutathione S-transferase catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls. J Bacteriol 188:4424-430. doi:10.-128/?JB.-1849-05 PubMed Central PubMed View Article
  Fujii T, Narikawa T, Takeda K, Kato J (2004) Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp. TF6. Biosci Biotechnol Biochem 68:2171-177. doi:10.-271/?bbb.-8.-171 PubMed View Article
  Kawakami N, Shoji O, Watanabe Y (2011) Use of perfluorocarboxylic acids to trick cytochrome P450BM3 into initiating the hydroxylation of gaseous alkanes. Angew Chem Int Ed 50:5315-318. doi:10.-002/?anie.-01007975 View Article
  Kim KK, Lee KC, Oh HM, Kim MJ, Eom MK, Lee JS (2008) Arthrobacter defluvii sp. nov., 4-chlorophenol-degrading bacteria isolated from sewage. Int J Syst Evol Microbiol 58:1916-921. doi:10.-099/?ijs.-.-5550-0 PubMed View Article
  Klerk H, van der Linden AC (1974) Bacterial degradation of cyclohexane participation of a co-oxidation reaction. Ant van Leeuw 40:7-5. doi:10.-007/?BF00394548 View Article
  Kubota M, Nodate M, Yasumoto-Hirose M, Uchiyama T, Kagami O, Shizuri Y, Misawa N (2005) Isolation and functional analysis of cytochrome P450 CYP153A genes from various environments. Biosci Biotechnol Biochem 69:2421-430. doi:10.-271/?bbb.-9.-421 PubMed View Article
  Kurth EG, Doughty DM, Bottomley PJ, Arp DJ, Sayavedra-Soto LA (2008) Involvement of BmoR and BmoG in n-alkane metabolism in Pseudomonas butanovora. Microbiology 154:139-47. doi:10.-099/?mic.-.-007/-12724-0 PubMed View Article
  Lee EH, Cho KS (2008) Characterization of cyclohexane and hexane degradation by Rhodococcus sp. EC1. Chemosphere 71:1738-744. doi:10.-016/?j.?chemosphere.-007.-2.-09 PubMed View Article
  Lee S, Oh H, Kim O (2013) Isolation and characterization of volatile organic compounds-degrading Bacillus strains from Loess. J Environ Protect 4:50-7. doi:10.-236/?jep.-013.-4A007 View Article
  Liu L, Schmid RD, Urlacher VB (2006) Cloning, expression, and characterization of a self-sufficient cytochrome P450 monooxygenase from Rhodococcus ruber DSM 44319. Appl Microbiol Biotechnol 72:876-82. doi:10.-007/?s00253-006-0355-0 PubMed View Article
  Maier T, F?rster HH, Asperger O, Hahn U (2001) Molecular characterization of the 56-kDa CYP153 from Acinetobacter sp. EB104. Biochem Biophys Res Commun 286:652-58. doi:10.-006/?bbrc.-001.-449 PubMed View Article
  Marks ME, Castro-Rojas CM, Teiling C, Du L, Kapatral V, Walunas TL, Crosson S (2010) The genetic basis of laboratory adaptation in Caulobacter crescentus. J Bacteriol 192:3678-688. doi:10.-128/?JB.-0255-10 PubMed Central PubMed View Article
  Masuda H (2009) Identification and characterization of monooxygenase enzymes involved in 1,4-dioxane degradation in Pseudonocardia sp. strain env478, Mycobacterium sp. strain env421, and Nocardia sp. strain env425. PhD thesis, University of New Jersey
  Mattes TE, Alexander AK, Richardson PM, Munk AC, Han CS, Stothard P, Coleman NV (2008) The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology. Appl Environ Microbiol 74:6405-416. doi:10.-128/?AEM.-0197-08 PubMed Central PubMed View Article
  McClay K, Boss C, Keresztes I, Steffan RJ (2005) Mutations of toluene-4-monooxygenase that alter regiospecificity of indole oxidation and lead to production of novel indigoid pigments. Appl Environ Mi
• 作者单位：Diego Salamanca (1) (3)
  Rohan Karande (2)
  Andreas Schmid (2)
  Daniel Dobslaw (1)
  
  1. Department of Biological Waste Air Purification, Institute of Sanitary Engineering, Water Quality and Solid Waste Management - University of Stuttgart, Bandt?le 2, 70569, Stuttgart, Germany
  3. Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany
  2. Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318, Leipzig, Germany
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols.