A whole cell biocatalyst for double oxidation of cyclooctane

详细信息    查看全文

• 作者：C. A. Müller ; A. M. Weingartner ; A. Dennig…
• 关键词：Alkanes ; Directed evolution ; Oxidation ; Oxidoreductases ; Cascade reaction ; Monooxygenase
• 刊名：Journal of Industrial Microbiology and Biotechnology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：43
• 期：12
• 页码：1641-1646
• 全文大小：618 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Chemistry
  Biotechnology
  Genetic Engineering
  Biochemistry
  Bioinformatics
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1476-5535
• 卷排序：43

文摘

A novel whole cell cascade for double oxidation of cyclooctane to cyclooctanone was developed. The one-pot oxidation cascade requires only a minimum of reaction components: resting E. coli cells in aqueous buffered medium (=catalyst), the target substrate and oxygen as environmental friendly oxidant. Conversion of cyclooctane was catalysed with high efficiency (50% yield) and excellent selectivity (>94%) to cyclooctanone. The reported oxidation cascade represents a novel whole cell system for double oxidation of non-activated alkanes including an integrated cofactor regeneration. Notably, two alcohol dehydrogenases from Lactobacillus brevis and from Rhodococcus erythropolis with opposite cofactor selectivities and one monooxygenase P450 BM3 were produced in a coexpression system in one single host. The system represents the most efficient route with a TTN of up to 24363 being a promising process in terms of sustainability as well.