Proteomics and Transcriptomics Analyses of Secretagogin Down-Regulation in Human Non-Functional Pituitary Adenomas

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• 作者：Xianquan Zhan ; Chheng-Orn Evans ; Nelson M. Oyesiku and Dominic M. Desiderio
• 关键词：secretagogin ; pituitary non ; functional adenoma ; proteome ; transcriptome ; two ; dimensional gel electrophoresis ; mass spectrometry ; oligonucleotide microarray
• 刊名：Pituitary
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：6
• 期：4
• 页码：189-202
• 全文大小：411 KB

文摘

In order to explore the presence of, and the potential role of, secretagogin in human pituitary adenomas, an analytical strategy that integrated comparative proteomics and comparative transcriptomics was used to detect the protein and the mRNA expression, respectively, of secretagogin in human non-functional pituitary adenomas compared to controls. Proteomics methods included two-dimensional gel electrophoresis, 2D gel image analysis, mass spectrometry [matrix-assisted laser desorption/ionization-time of flight-peptide mass fingerprinting (MALDI-TOF PMF) and liquid chromatography-electrospray ionization-quadrupole-ion trap tandem mass spectrometry (LC-ESI-Q-IT MS/MS)], and database analysis. Transcriptomics methods included the GeneChip microarray, image processing, and data analysis. The proteomics and transcriptomics data demonstrated that secretagogin was significantly down-regulated at the protein and mRNA levels, respectively, in the human non-functional (NF) pituitary adenomas (NF–, LH+, FSH+, and FSH+ + LH+). For the secretagogin protein, the expression level was NF– + + LH+ + + p – + + + LH+ + p