Subcellular localization of Arabidopsis thaliana heme-binding protein 1 (AtHBP1), AtHBP2 and AtHBP3

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• 作者：Kyoko Aizawa ; Shigekazu Takahashi…
• 关键词：Heme ; Heme binding protein ; p22HBP ; SOUL
• 刊名：Journal of Plant Biochemistry and Biotechnology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：25
• 期：2
• 页码：215-218
• 全文大小：405 KB
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• 作者单位：Kyoko Aizawa (1)
  Shigekazu Takahashi (2)
  Katsumi Nakayama (1)
  Hiroyuki Satoh (1)
  
  1. Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba, 274-8510, Japan
  2. Faculty of Life Sciences, Toyo University, Itakura, Gunma, 374-0193, Japan
  
• 刊物主题：Life Sciences, general; Plant Biochemistry; Protein Science; Receptors; Cell Biology;
• 出版者：Springer India
• ISSN：0974-1275

文摘

Heme is an essential pigment in various biochemical processes in plant cells, including oxidoreduction and electron transport. Plastids are known to be the site of heme synthesis in plant cells while the transport mechanism of heme from plastids to apoproteins that require heme in the cytosol and various organelles is still enigmatic. AtHBP1, AtHBP2, and AtHBP3, members of the SOUL/p22 heme binding protein family in Arabidopsis thaliana, are regarded as candidate cytosolic heme carrier proteins. However, the subcellular localization of the AtHBPs has not been determined. In this study, we investigated transcription levels and subcellular localization of the AtHBPs. Semi-quantitative RT-PCR analysis revealed that AtHBP1, AtHBP2 and AtHBP3 were transcribed in various tissues. The transcription levels of AtHBP1 and AtHBP3 in roots were lower than in other tissues. To reveal the subcellular localization of AtHBP1, AtHBP2 and AtHBP3, we constructed transgenic A. thaliana lines expressing Venus fluorescent protein fused, respectively, to the C-terminus of AtHBP1, AtHBP2 and AtHBP3. AtHBP1::Venus accumulated around the nucleus, AtHBP2::Venus was detected in the cytosol, and AtHBP3::Venus was observed in the peripheral region of the cell.