Analysis of the effect of soil saprophytic fungi on the eggs of Baylisascaris procyonis

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• 作者：Cristiana F. Cazapal-Monteiro ; José A. Hernández ; Fabián L. Arroyo…
• 关键词：Baylisascaris procyonis ; Raccoon ; Biological prevention ; Fungi ; Soil ; transmitted helminth
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：114
• 期：7
• 页码：2443-2450
• 全文大小：699 KB
• 参考文献：Arias MS, Cazapal-Monteiro CF, Suárez J, Miguélez S, Francisco I, Arroyo FL, Suárez JL, Paz-Silva A, Sánchez-Andrade R, Mendoza de Gives P (2013) Mixed production of filamentous fungal spores for preventing soil-transmitted helminth zoonoses: a preliminary analysis. Biomed Res Int. doi:10.-155/-013/-67876
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• 作者单位：Cristiana F. Cazapal-Monteiro (1)
  José A. Hernández (1)
  Fabián L. Arroyo (1)
  Silvia Miguélez (1)
  ángel Romasanta (1)
  Adolfo Paz-Silva (1)
  Rita Sánchez-Andrade (1)
  María S. Arias (1)
  
  1. Parasitology, Zoonoses and Epidemiology (COPAR, GI-2120), Animal Pathology Department, Veterinary Faculty, Santiago de Compostela University, 27002, Lugo, Spain
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Baylisascaris procyonis is a soil-transmitted helminth mainly found in raccoons (Procyon lotor) which can also affect other domestic and sylvatic animals, as well as humans, when the eggs released in the feces of parasitized raccoons are accidentally ingested. Three assays have been conducted to assess the effect of three saprophytic fungi, Mucor circinelloides, Paecilomyces lilacinus, and Verticillium sp., on the eggs of B. procyonis. Firstly, their ovicidal effect was in vitro ascertained by placing 1?mL with 2?×-06 spores of each fungus in Petri plates with water-agar (2?%) and simultaneously adding 200 eggs of Baylisascaris/plate. Two in vivo probes were carried out, by spraying the fungal spores (3?mL containing about 2?×-06 spores/mL) on the feces of raccoons and coatis (Nasua narica) passing eggs of B. procyonis in a zoological park; the other assay consisted of evaluating the activity of the fungi after adding sand to fecal samples from raccoons. An ovicidal type 3 activity characterized by morphological damage of the eggshell with hyphal penetration, internal egg colonization, and embryo alteration was observed for all the tested fungi. In the plate assays, viability of Baylisascaris eggs reduced significantly by 53-9?% with Mucor, 45-2?% with Paecilomyces, and 52-7?% with Verticillium. A similar ovicidal effect was detected in the feces with sand. These results demonstrate the usefulness of spraying spores of M. circinelloides, Pa. lilacinus, or Verticillium sp. on the feces of animals infected by Baylisascaris to decrease the numbers of viable eggs and, thus, the risk of infection.