Effect of high versus low doses of fat and vitamin A dietary supplementation on fatty acid composition of phospholipids in mice

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• 作者：Kathrin Weiss (1)
  Johanna Mihy (1)
  Gerhard Liebisch (2)
  Tam Marosvlgyi (3)
  Ada L. Garcia (4)
  Gerd Schmitz (2)
  Tam Decsi (3)
  Ralph Rl (1)
  
• 关键词：Vitamin A ; Poly ; unsaturated fatty acids ; Desaturase ; Elongase ; Phospholipids
• 刊名：Genes & Nutrition
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：9
• 期：1
• 全文大小：
• 作者单位：Kathrin Weiss (1)
  Johanna Mihy (1)
  Gerhard Liebisch (2)
  Tam Marosvlgyi (3)
  Ada L. Garcia (4)
  Gerd Schmitz (2)
  Tam Decsi (3)
  Ralph Rl (1)
  
  1. Department of Biochemistry and Molecular Biology, Medical and Health Science Center, University of Debrecen, Nagyerdei Krt. 98, Debrecen, 4032, Hungary
  2. Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany
  3. Department of Paediatrics, University of Ps, Pecs, Hungary
  4. Human Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
  
• ISSN：1865-3499

文摘

Dietary fat and vitamin A provide important precursors for potent bioactive ligands of nuclear hormone receptors, which regulate various enzymes involved in lipid homeostasis, metabolism and inflammation. We determined the effects of dietary fat and dietary vitamin A on hepatic expression of two fatty acid metabolizing enzymes, elongase 6 (ELOVL6) and stearoyl-coenzyme A desaturase 1 (SCD1) and the concentration of saturated fatty acids (SAFA) and monounsaturated fatty acid (MUFA) of phospholipids in serum and liver. Mice (n=6) were fed 4weeks with diets containing 2, 5 and 25% of fat or vitamin A (0, 2,500 and 326,500 RE/kg as retinyl palmitate). MUFAs and SAFAs were measured using GC and ESIS/MS. Hepatic expression of metabolizing enzymes was determined using QRT-PCR. ELOVL6 was significantly down-regulated in response to a high-fat diet (p<0.001) and significantly up-regulated in response to low-fat diet (p<0.05). SCD1 expression was significantly lower in high- versus low-fat diet (p<0.05). The vitamin A content in the diet did not influence the hepatic expression of both enzymes. In plasma, the amounts of MUFAs bound to phospholipids significantly decreased in response to a high-fat diet and increased after a low-fat diet. This tendency was also observed in the liver for various phospholipids sub-classes. In summary, this study shows that fat content in the diet has a stronger impact than the content of vitamin A on hepatic gene expression of SCD1 and ELOVL6 and thereby on MUFA and SAFA concentrations in liver and plasma.