Vitamin E prevents the age-dependent and palmitate-induced disturbances of sphingolipid turnover in liver cells

详细信息    查看全文

• 作者：Nataliya A. Babenko (1) babenko@univer.kharkov.ua
  Loay Kh. M. Hassouneh (2)
  Vitalina S. Kharchenko (1)
  Vladimir V. Garkavenko (1)
• 关键词：Old age – ; Liver cells – ; Vitamin E – ; α ; Tocopherol – ; Palmitic acid – ; Ceramide – ; Sphingomyelinases
• 刊名：AGE
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：34
• 期：4
• 页码：905-915
• 全文大小：367.6 KB
• 参考文献：1. Ayasolla K, Khan M, Singh AK, Singh I (2004) Inflammatory mediator and beta-amyloid (25–35)-induced ceramide generation and iNOS expression are inhibited by vitamin E. Free Radic Biol Med 37:325–338. doi:
  2. Babenko NA, Semenova YA (2010) Effects of long-term fish oil-enriched diet on the sphingolipid metabolism in brain of old rats. Exp Gerontol 45:375–380. doi:
  3. Babenko N, Shakhova E (2006) Effects of Chamomilla recutita flavonoids on age-related liver sphingolipid turnover in rats. Exp Geront 41:32–39. doi:
  4. Babenko N, Shakhova E (2008) Effects of flavonoids on sphingolipid turnover in the toxin-damaged liver and liver cells. Lipids Health Dis 7:1. doi:
  5. Bai Y, Wang J, Shan H, Lu Y, Zhang Y, Luo X, Yang B, Wang Z (2007) Sphingolipid metabolite ceramide causes metabolic perturbation contributing to HERG K + channel dysfunction. Cell Physiol Biochem 20:429–440. doi:
  6. Bansal AK, Bansal M, Soni G, Bhatnagar D (2005) Protective role of vitamin E pre-treatment on N-nitrosodiethylamine induced oxidative stress in rat liver. Chem Biol Interect 156:101–111. doi:
  7. Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468
  8. Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888
  9. Bl谩zquez C, Galve-Roperh I, Guzm谩n M (2000) De novo-synthesized ceramide signals apoptosis in astrocytes via extracellular signal-regulated kinase. FASEB J 14:2315–2322
  10. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
  11. Charruyer A, Grazide S, Bezombes C, Muller S, Laurent G, Jaffrezou JP (2005) UV-C light induces raft-associated acid sphingomyelinase and JNK activation and translocation independently on a nuclear signal. J Biol Chem 280:19196–19204. doi:
  12. Crivello NA, Rosenberg IH, Dallal GE, Bielinski D, Joseph JA (2005) Age-related changes in neutral sphingomyelin-specific phospholipase C activity in striatum, hippocampus, and frontal cortex: implication for sensitivity to stress and inflammation. Neurochem Int 47:573–579. doi:
  13. Cutler RG, Mattson MP (2001) Sphingomyelin and ceramide as regulators of development and lifespan. Mech Ageing Dev 122:895–908. doi:
  14. Denis U, Lecomte M, Paget C, Ruggiero D, Wiernsperger N, Lagarde M (2002) Advanced glycation end-products induce apoptosis of bovine retinal pericytes in culture: involvement of diacylglycerol/ceramide production and oxidative stress induction. Free Radic Biol Med 33:236–247. doi:
  15. Dumitru CA, Gulbins E (2006) TRAIL activates acid sphingomyelinase via a redox mechanism and releases ceramide to trigger apoptosis. Oncogene 25:5612–5625. doi:
  16. Freisleben H-Y, Mentrup E (1992) Preparation and properties of liposomes containing vitamin E. In: Packes L, Fuchs J (eds) Vitamin E in Health and Disease. Marsel Dekker, New York, pp 193–206
  17. Garcia-Ruiz C, Mari M, Morales A, Colell A, Ardite E, Fernandez-Checa JC (2000) Human placenta sphingomyelinase, induces oxidative stress, glutathione depletion, and apoptosis in rat hepatocytes. Hepatology 32:56–65. doi:
  18. Grether-Beck S, Bonizzi G, Schmitt-Brenden H, Felsner I, Timmer A, Sies H, Johnson JP, Piette J, Krutmann J (2000) Non-enzymatic triggering of the ceramide signaling cascade by solar UVA radiation. EMBO J 19:5793–5800. doi:
  19. Grether-Beck S, Timmer A, Felsner I, Brenden H, Brammertz D, Krutmann J (2005) Ultraviolet A-induced signaling involves a ceramide-mediated autocrine loop leading to ceramide de novo synthesis. J Invest Dermatol 125:545–553. doi:
  20. Hatanaka Y, Fujii J, Fukutomi T, Watanabe T, Che W, Sanada Y, Igarashi Y, Taniguchi N (1998) Reactive oxygen species enhances the induction of inducible nitric oxide synthase by sphingomyelinase in RAW264.7 cells. Biochim Biophys Acta 1393:203–210. doi:
  21. Holland WL, Brozinick JT, Wang LP, Hawkins ED, Sargent KM, Liu Y, Narra K, Hoehn KL, Knotts TA, Siesky A, Nelson DH, Karathanasis SK, Fontenot GK, Birnbaum MJ, Summers SA (2007) Inhibition of ceramide synthesis ameliorates glucocorticoid-, saturated-fat-, and obesity-induced insulin resistance. Cell Metab 5:167–179. doi:
  22. Ichi I, Kamikawa C, Nakagawa T, Kobayashi K, Kataoka R, Nagata E, Kitamura Y, Nakazaki C, Matsura T, Kojo S (2009) Neutral sphingomyelinase-induced ceramide accumulation by oxidative stress during carbon tetrachloride intoxication. Toxicology 261:33–40. doi:
  23. Kavok NS, Krasilnikova OA, Babenko NA (2003) Increase in diacylglycerol production by liver and liver nuclei at old age. Exp Gerontol 38:441–447. doi:
  24. Lauter CJ, Trams EG (1962) A spectrophotometric determination of sphingosine. J Lipid Res 3:135–138
  25. Lightle SA, Oakley JI, Nikolova-Karakashian MN (2000) Activation of sphingolipid turnover and chronic generation of ceramide and sphingosine in liver during aging. Mech Ageing Dev 120:111–125
  26. Listenberger LL, Han X, Lewis SE, Cases S, Farese RV Jr, Ory DS, Schaffer JE (2003) Triglyceride accumulation protects against fatty acid-induced lipotoxicity. Proc Natl Acad Sci USA 100:3077–3082. doi:
  27. Liu B, Hannun YA (1997) Inhibition of the neutral magnesium-dependent sphingomyelinase by glutathione. J Biol Chem 272:16281–16287. doi:
  28. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin–phenol reagents. J Biol Chem 193:265–275
  29. Mart铆n SF, Navarro F, Forthoffer N, Navas P, Villalba JM (2001) Neutral magnesium-dependent sphingomyelinase from liver plasma membrane: purification and inhibition by ubiquinol. J Bioenerg Biomembr 33:143–153
  30. Massip L, Garand C, Paquet ER, Cogger VC, O’Reilly JN, Tworek L, Hatherell A, Taylor CG, Thorin E, Zahradka P, Le Couteur DG, Lebel M (2010) Vitamin C restores healthy aging in a mouse model for Werner syndrome. FASEB J 24:158–172. doi:
  31. Mazi猫re C, Conte MA, Leborgne L, Levade T, Hornebeck W, Santus R, Mazi猫re JC (2001) UVA radiation stimulates ceramide production: relationship to oxidative stress and potential role in ERK, JNK, and p38 activation. Biochem Biophys Res Commun 281:289–294. doi:
  32. Merrill AH Jr, Nixon DW, Williams RD (1985) Activities of serine palmitoyltransferase (3-ketosphinganine synthase) in microsomes from different rat tissues. J Lipid Res 26:617–622
  33. Merrill AH Jr, Lingrell S, Wang E, Nikolova-Karakashian M, Vales TR, Vance DE (1995) Sphingolipid biosynthesis de novo by rat hepatocytes in culture. J Biol Chem 270:13834–13841. doi:
  34. Nagle CA, Klett EL, Coleman RA (2009) Hepatic triacylglycerol accumulation and insulin resistance. J Lipid Res 50:S74–S79. doi:
  35. Nakamura S, Takamura T, Matsuzawa-Nagata N, Takayama H, Misu H, Noda H, Nabemoto S, Kurita S, Ota T, Ando H, Miyamoto K, Kaneko S (2009) Palmitate induces insulin resistance in H4IIEC3 hepatocytes through reactive oxygen species produced by mitochondria. J Biol Chem 284:14809–14818. doi:
  36. Nakata K, Kawase M, Ogino S, Kinoshita C, Murata H, Sakaue T, Ogata T, Ohmori S (1996) Effects of age on levels of cysteine, glutathione and related enzyme activities in livers of mice and rats and an attempt to replenish hepatic glutathione level of mouse with cysteine derivatives. Mech Ageing Dev 90:195–207. doi:
  37. Perry DK, Carton J, Shah AK, Meredith F, Uhlinger DJ, Hannun YA (2000) Serine palmitoyltransferase regulates de novo ceramide generation during etoposide-induced apoptosis. J Biol Chem 275:9078–9084. doi:
  38. Post A, Holsboer F, Behl C (1998) Induction of NF-kappaB activity during haloperidol-induced oxidative toxicity in clonal hippocampal cells: suppression of NF-kappaB and neuroprotection by antioxidants. J Neurosci 18:8236–8246
  39. Rutkute K, Asmis RH, Nikolova-Karakashian MN (2007) Regulation of neutral sphingomyelinase-2 by GSH: a new insight to the role of oxidative stress in aging-associated inflammation. J Lipid Res 48:2443–2452. doi:
  40. Sanvicens N, Cotter TG (2006) Ceramide is the key mediator of oxidative stress-induced apoptosis in retinal photoreceptors. J Neurochem 98:1432–1444. doi:
  41. Sathishkumar S, Boyanovsky B, Karakashian AA, Rozenova K, Giltiay NV, Kudrimoti M, Mohiuddin M, Ahmed MM, Nikolova-Karakashian M (2005) Elevated sphingomyelinase activity and ceramide concentration in serum of patients undergoing high dose spatially fractionated radiation treatment: implications for endothelial apoptosis. Cancer Biol Ther 4:979–986. doi:
  42. Scheel-Toellner D, Wang K, Craddock R, Webb PR, McGettrick HM, Assi LK, Parkes N, Clough LE, Gulbins E, Salmon M, Lord JM (2004) Reactive oxygen species limit neutrophil life span by activating death receptor signaling. Blood 104:2557–2564. doi:
  43. Strivastava S, Chan C (2007) Hydrogen peroxide and hydroxyl radicals mediate palmitate-induced cytotoxicity to hepatoma cells: relation to mitochondrial permeability transition. Free Radical Res 41:38–49. doi:
  44. Su JF, Guo CJ, Wei JY, Yang JJ, Jiang YG, Li YF (2003) Protection against hepatic ischemia-reperfusion injury in rats by oral pretreatment with guercetin. Biomed Environ Sci 16:1–8
  45. Watson ML, Coghlan M, Hundal HS (2009) Modulating serine palmitoyl transferase (SPT) expression and activity unveils a critical role in lipid-induced insulin resistance in rat skeletal muscle cells. Biochem J 417:791–801. doi:
  46. Wei Y, Wang D, Topczewski F, Pagliassotti MJ (2006) Saturated fatty acids induce endoplasmic reticulum stress and apoptosis independently of ceramide in liver cells. Am J Physiol Endocrinol Metab 291:E275–E281. doi:
  47. Yamagata K, Ichinose S, Tagawa C, Tagami M (2009) Vitamin E regulates SMase activity, GSH levels, and inhibits neuronal death in stroke-prone spontaneously hypertensive rats during hypoxia and reoxygenation. J Exp Stroke Transl Med 2:2. doi:
  48. Zeidan YH, Hannun YA (2007) Activation of acid sphingomyelinase by protein kinase Cδ-mediated phosphorylation. J Biol Chem 282:11549–11561. doi:
• 作者单位：1. Department of Physiology of Ontogenesis, Institute of Biology, Kharkov Karazin National University, 4 Svobody pl, 61077 Kharkov, Ukraine2. Department of Biology, Faculty of Science, Hail University, P.O. Box 2440, Hail, Kingdom of Saudi Arabia
• ISSN：1574-4647

文摘

Sphingolipid turnover has been shown to be activated at old age and in response to various stress stimuli including oxidative stress. Reduction of vitamin E content in the liver under the pro-oxidant action is associated with enhanced sphingolipid turnover and ceramide accumulation in hepatocytes. In the present paper, the correction of sphingolipid metabolism in the liver cells of old rats and in the palmitate-treated young hepatocytes using α-tocopherol has been investigated. 3- and 24-month-old rats, [14 C]palmitic acid, [methyl−14 C-choline]sphingomyelin (SM), and [14 C]serine were used. α-Tocopherol administration to old rats or addition to the culture medium of old liver slices or hepatocytes prevented age-dependent increase of ceramide synthesis and lipid accumulation, and increased SM content in liver tissue and cells. α-Tocopherol treatment of old cells decreased the neutral and acid sphingomyelinase (SMase) activities in hepatocytes and serine palmitoyl transferase activity in the liver cell microsomes. Effect of α- or γ-tocopherol, but not of δ-tocopherol, on the newly synthesized ceramide content in old cells was correlated with the action of inhibitor of serine palmitoyl transferase (SPT) activity (myriocin) and SMase inhibitors (glutathione, imipramine). Addition of α-tocopherol as well as myriocin to the culture medium of young hepatocytes, treated by palmitate, abolished ceramide accumulation and synthesis. The data obtained demonstrate that α-tocopherol normalized elevated ceramide content in the old liver cells via inhibition of acid and neutral SMase activities and lipid synthesis de novo. α-Tocopherol, reducing ceramide synthesis, prevented palmitate-induced aging-like ceramide accumulation in young liver cells.