Quantification of Salmonella by 5'-Nuclease Real-Time Polymerase Chain Reaction Targeted to fimC Gene

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• 作者：?ubica Piknov¨¢ ; Eva Kacl¨ªkov¨¢ ; Domenico Pangallo ; Bystr¨ªk Polek and Tom¨¢? Kuchta
• 刊名：Current Microbiology
• 出版年：2005
• 出版时间：January 2005
• 年：2005
• 卷：50
• 期：1
• 页码：38-42
• 全文大小：95 KB

文摘

A new 5-nuclease real-time polymerase chain reaction (PCR) system for the quantification of Salmonella enterica was developed, with primers and the probe oriented to a Salmonella-specific region of the fimC gene. The PCR system was specific and sensitive, its inclusivity was 100 % (determined by the analysis of 53 strains of Salmonella belonging to 38 serovars) and its exclusivity was 100 % (determined by the analysis of 49 non-Salmonella strains). For quantification purposes, calibration lines were constructed for three Salmonella strains belonging to three serotypes. These calibration lines were linear (r0.99) in the range from 103 to 107 CFU/mL and practically identical in terms of very similar slopes and x-intercepts. Escherichia coli (106 CFU/mL) and Citrobacter freundii (106 CFU/mL) had no effect on Salmonella quantification by the system.