Characterization of a multiprotein complex involved in excitation-transcription coupling of skeletal muscle
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- 作者：Manuel Arias-Calderón ; Gonzalo Almarza ; Alexis Díaz-Vegas…
- 关键词：Multiprotein complex ; Excitation ; transcription coupling ; Dihydropyridine receptor ; Nucleotide receptors ; Pannexin 1 ; Skeletal muscle plasticity
- 刊名：Skeletal Muscle
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：6
- 期：1
- 全文大小：2,918 KB
- 刊物主题：Cell Biology; Developmental Biology; Biochemistry, general; Systems Biology; Biotechnology;
- 出版者：BioMed Central
- ISSN：2044-5040
文摘
Background Electrical activity regulates the expression of skeletal muscle genes by a process known as “excitation-transcription” (E-T) coupling. We have demonstrated that release of adenosine 5′-triphosphate (ATP) during depolarization activates membrane P2X/P2Y receptors, being the fundamental mediators between electrical stimulation, slow intracellular calcium transients, and gene expression. We propose that this signaling pathway would require the proper coordination between the voltage sensor (dihydropyridine receptor, DHPR), pannexin 1 channels (Panx1, ATP release conduit), nucleotide receptors, and other signaling molecules. The goal of this study was to assess protein-protein interactions within the E-T machinery and to look for novel constituents in order to characterize the signaling complex.