Inhaled corticosteroid use is associated with increased circulating T regulatory cells in children with asthma

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• 作者：Anne Marie Singh (1) (5)
  Paul Dahlberg (4)
  Kristjan Burmeister (4)
  Michael D Evans (2)
  Ronald Gangnon (2) (3)
  Kathy A Roberg (1)
  Christopher Tisler (1)
  Douglas DaSilva (1)
  Tressa Pappas (1)
  Lisa Salazar (1)
  Robert F Lemanske Jr (1) (4)
  James E Gern (1) (4)
  Christine M Seroogy (1)
  
• 关键词：Asthma ; CD127 ; Foxp3 ; Inhaled corticosteroids ; T regulatory cell
• 刊名：Clinical and Molecular Allergy
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：11
• 期：1
• 全文大小：234KB
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• 作者单位：Anne Marie Singh (1) (5)
  Paul Dahlberg (4)
  Kristjan Burmeister (4)
  Michael D Evans (2)
  Ronald Gangnon (2) (3)
  Kathy A Roberg (1)
  Christopher Tisler (1)
  Douglas DaSilva (1)
  Tressa Pappas (1)
  Lisa Salazar (1)
  Robert F Lemanske Jr (1) (4)
  James E Gern (1) (4)
  Christine M Seroogy (1)
  
  1. Departments of Pediatrics, University of Wisconsin, 1111 Highland Avenue, 4139 WIMR, Madison, WI, 53705-2275, USA
  5. Northwestern Feinberg School of Medicine, Chicago, IL, USA
  4. Departments of Medicine, University of Wisconsin, Madison, WI, USA
  2. Departments of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI, USA
  3. Departments of Population Health Sciences, University of Wisconsin, Madison, WI, USA
  
• ISSN：1476-7961

文摘

Background T regulatory (Treg) cells are important in balancing immune responses and dysregulation of Treg cells has been implicated in the pathogenesis of multiple disease states including asthma. In this study, our primary aim was to determine Treg cell frequency in the peripheral blood of children with and without asthma. The secondary aim was to explore the association between Treg cell frequency with allergen sensitization, disease severity and medication use. Methods Peripheral blood mononuclear cells from healthy control subjects (N--3) and asthmatic children of varying disease severity (N--6) were characterized by multi-parameter flow cytometry. Results Our findings demonstrate that children with asthma had a significantly increased frequency of Treg cells compared to children without asthma. Using a multivariate model, increased Treg cell frequency in children with asthma was most directly associated with inhaled corticosteroid use, and not asthma severity, allergic sensitization, or atopic status of the asthma. Conclusion We conclude that low dose, local airway administration of corticosteroids is sufficient to impact the frequency of Treg cells in the peripheral blood. These data highlight the importance of considering medication exposure when studying Treg cells and suggest inhaled corticosteroid use in asthmatics may improve disease control through increased Treg cell frequency.