文摘

We have developed a screen-printed electrochemical electrode (SPE) for paraoxon based on its inhibitory effect on the enzyme butyrylcholinesterase (BChE). The electrode was first modified by drop casting with a dispersion of carbon black nanoparticles (CBNPs) in a dimethylformamide-water mixture, and BChE was then immobilized on the surface by cross-linking. The resulting biosensor was exposed to standard solutions of paraoxon, and the enzymatic hydrolysis of butyrylthiocholine over time was determined measuring the enzymatic product thiocholine at a working voltage of +300?mV. The enzyme inhibition is linearly related to the concentration of paraoxon up to 30?μg?L?, and the detection limit is 5?μg?L?. The biosensor is stable for up to 78?days of storage at room temperature under dry conditions. It was applied to determined paraoxon in spiked waste water samples. The results underpin the potential of the use of CBNPs in electrochemical biosensors and also demonstrate that they represent a viable alternative to other carbon nanomaterials such as carbon nanotubes or graphene, and with the advantage of being very affordable. Graphical Abstract A screen-printed electrode (SPE) for paraoxon detection at ppb levels based on its inhibitory effect on the enzyme butyrylcholinesterase was developed. The measurement is characterised by absence of fouling problem due to the use of carbon black nanoparticles.