Detection of the camellia root-knot nematode Meloidogyne camelliae Golden in Japanese camellia bonsai imported into Italy: integrative diagnosis, parasitic habits and molecular phylogeny

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• 作者：Nicola Trisciuzzi (1)
  Alberto Troccoli (2)
  Nicola Vovlas (2)
  Carolina Cantalapiedra-Navarrete (3)
  Juan E. Palomares-Rius (3) (4)
  Pablo Castillo (3)
  
• 关键词：Alien nematode pest interception ; Histopathology ; mtDNA ; Phylogeny ; rDNA
• 刊名：European Journal of Plant Pathology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：138
• 期：2
• 页码：231-235
• 全文大小：255 KB
• 作者单位：Nicola Trisciuzzi (1)
  Alberto Troccoli (2)
  Nicola Vovlas (2)
  Carolina Cantalapiedra-Navarrete (3)
  Juan E. Palomares-Rius (3) (4)
  Pablo Castillo (3)
  
  1. Centro di Ricerca, Sperimentazione e Formazione in Agricoltura (CRSFA) “Basile Caramia- Area Servizi -Laboratorio Fitopatologico, Via Cisternino, 281, Locorotondo, BA, Italy
  2. Istituto per la Protezione delle Piante (IPP), Consiglio Nazionale delle Ricerche (CNR), U.O.S. di Bari, Via G. Amendola 122/D, 70126, Bari, Italy
  3. Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Apdo. 4084, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14080, Córdoba, Spain
  4. Department of Forest Pathology, Forestry and Forest Products Research Institute (FFPRI), Tsukuba, 305-8687, Ibaraki, Japan
  
• ISSN：1573-8469

文摘

The camellia root-knot nematode Meloidogyne camelliae was detected in Italy parasitizing roots of Japanese camellia (Camellia sasanqua) flowering bonsai trees imported from Japan, and it represents a new record for Europe. Morphology and morphometrical traits analysis of the intercepted population of M. camelliae were in agreement with those of the original description of the species, except for some minor differences in second-stage juvenile stylet length and c ratio, spicules and gubernaculum in males, which may be a result of intraspecific variability. Integrative molecular data for this species were obtained using D2–D3 expansion segments of 28S rDNA, ITS1–rDNA, and the protein–coding mitochondrial gene COI. The phylogenetic relationships of this species with other representatives of Meloidogyne spp. using D2-D3 expansion segments and ITS1 indicated that M. camelliae clustered together with M. artiellia and M. baetica. Histological observations of C. sasanqua feeder roots infected by M. camelliae reveal slight swellings of the root feeding sites, and most egg–laying females protruding from the root. The anatomical alterations at feeding sites consist of 3- hypertrophied, polinucleate giant cells/female, but no hyperplasia was induced unlike what commonly occurs for most members of the genus.