Production, Partial Purification and Characterization of Enzyme Cocktail from Trichoderma citrinoviride AUKAR04 Through Solid-State Fermentation

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• 作者：Karthik Periyasamy ; Laishram Santhalembi…
• 关键词：Xylanase ; CMCase ; β ; 1 ; 3 ; Glucanase ; Solid ; state fermentation ; Three ; phase partitioning ; Trichoderma sp.
• 刊名：Arabian Journal for Science and Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：42
• 期：1
• 页码：53-63
• 全文大小：
• 刊物类别：Engineering
• 刊物主题：Engineering, general; Science, Humanities and Social Sciences, multidisciplinary;
• 出版者：Springer Berlin Heidelberg
• ISSN：2191-4281
• 卷排序：42

文摘

A strain of Trichoderma citrinoviride AUKAR04 was identified on the basis of morphological and 5.8S ribosomal RNA sequencing [GenBank: KF698728]. It produces cocktail of enzymes such as xylanase (55,000 IU gds−1), CMCase (375 IU gds−1) and β-1,3-glucanase (695 IU gds−1) after 72 h under solid-state fermentation. These enzymes were partially purified by a three-phase partitioning method, which recovered the maximum activities of xylanase (99.8 %) with 5.7-fold, CMCase (96.5 %) with 5.5-fold and β-1,3-glucanase (98.4 %) with 5.6-fold purification. The maximum activity of xylanase was observed at pH 5.0, CMCase at pH 5.0–6.0 and β-1,3-glucanase at pH 6.0. Optimum temperature of xylanase and β-1,3-glucanase was found to be at 50 °C, while for CMCase was at 60 °C. The activities of these enzymes were enhanced by Mg2+ and Mn2+ ions. Eucalyptus pulp fiber was incubated for 14 h with the enzyme cocktail. Xylanase hydrolyzed the pulp to yield arabinose (475 mg L−1) and xylose (1795 mg L−1), CMCase and β-1,3-glucanase released glucose (18763 mg L−1). The length of fiber was reduced from 0.881 to 0.056 mm. This is indicative of the potential application on bioconversion of lignocellulosic biomass into fermentable sugars by the enzyme cocktail produced from T. citrinoviride AUKAR04 for sustainable production of bioethanol.