Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

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• 作者：Bhakti R. Pathak ; Ananya A. Breed ; Snehal Apte…
• 关键词：CRISP ; 3 ; Prostate cancer ; Prognosis ; shRNA ; PSA ; ANXA1
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：411
• 期：1-2
• 页码：11-21
• 全文大小：1,521 KB
• 参考文献：1.Obort AS, Ajadi MB, Akinloye O (2013) Prostate-specific antigen: any successor in sight? Rev Urol 15:97–107PubMedCentral PubMed
  2.Kosari F, Asmann YW, Cheville JC, Vasmatzis G (2002) Cysteine-rich secretory protein-3: a potential biomarker for prostate cancer. Cancer Epidemiol Biomarkers Prev 11:1419–1426PubMed
  3.Bjartell AS, Al-Ahmadie H, Serio AM, Eastham JA, Eggener SE, Fine SW et al (2007) Association of cysteine-rich secretory protein 3 and beta-microseminoprotein with outcome after radical prostatectomy. Clin Cancer Res 13:4130–4138PubMedCentral CrossRef PubMed
  4.Dahlman A, Edsjö A, Halldén C, Persson JL, Fine SW, Lilja H et al (2010) Effect of androgen deprivation therapy on the expression of prostate cancer biomarkers MSMB and MSMB-binding protein CRISP3. Prostate Cancer Prostatic Dis 13:369–375. doi:10.​1038/​pcan.​2010.​25 CrossRef PubMed
  5.Grupp K, Kohl S, Sirma H, Simon R, Steurer S, Becker A et al (2013) Cysteine-rich secretory protein 3 overexpression is linked to a subset of PTEN-deleted ERG fusion-positive prostate cancers with early biochemical recurrence. Mod Pathol 26:733–742. doi:10.​1038/​modpathol.​2012.​206 CrossRef PubMed
  6.Al Bashir S, Alshalalfa M, Hegazy SA, Dolph M, Donnelly B, Bismar TA (2014) Cysteine-rich secretory protein 3 (CRISP3), ERG and PTEN define a molecular subtype of prostate cancer with implication to patients’ prognosis. J Hematol Oncol 7:7–21. doi:10.​1186/​1756-8722-7-21 CrossRef
  7.Gibbs GM, Roelants K, O’Bryan MK (2008) The CAP superfamily: cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins—roles in reproduction, cancer, and immune defense. Endocr Rev 7:865–897. doi:10.​1210/​er.​2008-0032 CrossRef
  8.Krätzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD (1996) The human cysteine-rich secretary protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3. Eur J Biochem 236:827–836CrossRef PubMed
  9.Kjeldsen L, Cowland JB, Johnsen AH, Borregaard N (1996) SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein. FEBS Lett 380:246–250CrossRef PubMed
  10.Niderman T, Genetet I, Bruyère T, Gees R, Stintzi A, Legrand M et al (1995) Pathogenesis-related PR-1 proteins are antifungal. Isolation and characterization of three 14-kilodalton proteins of tomato and of a basic PR-1 of tobacco with inhibitory activity against Phytophthora infestans. Plant Physiol 108:17–27PubMedCentral CrossRef PubMed
  11.Lee U, Nam YR, Ye JS, Lee KJ, Kim N, Joo CH (2014) Cysteine-rich secretory protein 3 inhibits hepatitis C virus at the initial phase of infection. Biochem Biophys Res Commun 450:1076–1082. doi:10.​1016/​j.​bbrc.​2014.​06.​106 CrossRef PubMed
  12.Brown RL, Lynch LL, Haley TL, Arsanjani R (2003) Pseudechetoxin binds to the pore turret of cyclic nucleotide-gated ion channels. J Gen Physiol 122:749–760PubMedCentral CrossRef PubMed
  13.Gibbs GM, Scanlon MJ, Swarbrick J, Curtis S, Gallant E, Dulhunty AF et al (2006) The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. J Biol Chem 281:4156–4163CrossRef PubMed
  14.Udby L, Lundwall A, Johnsen AH, Fernlund P, Valtonen-André C, Blom AM et al (2005) beta-Microseminoprotein binds CRISP-3 in human seminal plasma. Biochem Biophys Res Commun 333:555–561PubMedCentral CrossRef PubMed
  15.Nam RK, Reeves JR, Toi A, Dulude H, Trachtenberg J, Emami M et al (2006) A novel serum marker, total prostate secretory protein of 94 amino acids, improves prostate cancer detection and helps identify high grade cancers at diagnosis. J Urol 175:1291–1297CrossRef PubMed
  16.Pathak BR, Breed AA, Nakhawa VH, Jagtap DD, Mahale SD (2010) Growth inhibition mediated by PSP94 or CRISP-3 is prostate cancer cell line specific. Asian J Androl 12:677–689. doi:10.​1038/​aja.​2010.​56 PubMedCentral CrossRef PubMed
  17.Perretti M, D’Acquisto F (2009) Annexin A1 and glucocorticoids as effectors of the resolution of inflammation. Nat Rev Immunol 9:62–70. doi:10.​1038/​nri2470 CrossRef PubMed
  18.Bjartell A, Johansson R, Björk T, Gadaleanu V, Lundwall A, Lilja H et al (2006) Immunohistochemical detection of cysteine-rich secretory protein 3 in tissue and in serum from men with cancer or benign enlargement of the prostate gland. Prostate 66:591–603CrossRef PubMed
  19.Balk SP, Ko YJ, Bubley GJ (2003) Biology of prostate-specific antigen. J Clin Oncol 21:383–391CrossRef PubMed
  20.Lange PH, Ercole CJ, Lightner DJ, Fraley EE, Vessella R (1989) The value of serum prostate specific antigen determinations before and after radical prostatectomy. J Urol 141:873–879PubMed
  21.Banefelt J, Liede A, Mesterton J, Stålhammar J, Hernandez RK, Sobocki P et al (2014) Survival and clinical metastases among prostate cancer patients treated with androgen deprivation therapy in Sweden. Cancer Epidemiol 38:442–447. doi:10.​1016/​j.​canep.​2014.​04.​007 CrossRef PubMed
  22.Briganti A, Suardi N, Gallina A, Abdollah F, Novara G, Ficarra V et al (2014) Predicting the risk of bone metastasis in prostate cancer. Cancer Treat Rev 40:3–11. doi:10.​1016/​j.​ctrv.​2013.​07.​001 CrossRef PubMed
  23.Culig Z, Hobisch A, Cronauer MV, Cato AC, Hittmair A, Radmayr C et al (1993) Mutant androgen receptor detected in an advanced-stage prostatic carcinoma is activated by adrenal androgens and progesterone. Mol Endocrinol 7:1541–1550PubMed
  24.Koivisto PA, Helin HJ (1999) Androgen receptor gene amplification increases tissue PSA protein expression in hormone-refractory prostate carcinoma. J Pathol 189:219–223CrossRef PubMed
  25.Yeung F, Li X, Ellett J, Trapman J, Kao C, Chung LW (2000) Regions of prostate-specific antigen (PSA) promoter confer androgen-independent expression of PSA in prostate cancer cells. J Biol Chem 275:40846–40855CrossRef PubMed
  26.Webber MM, Waghray A, Bello D (1995) Prostate-specific antigen, a serine protease, facilitates human prostate cancer cell invasion. Clin Cancer Res 1:1089–1094PubMed
  27.Cumming AP, Hopmans SN, Vukmirović-Popović S, Duivenvoorden WC (2011) PSA affects prostate cancer cell invasion in vitro and induces an osteoblastic phenotype in bone in vivo. Prostate Cancer Prostatic Dis 14:286–294. doi:10.​1038/​pcan.​2011.​34 CrossRef PubMed
  28.Lim LH, Pervaiz S (2007) Annexin 1: the new face of an old molecule. FASEB J 21:968–975CrossRef PubMed
  29.Christmas P, Callaway J, Fallon J, Jones J, Haigler HT (1991) Selective secretion of annexin 1, a protein without a signal sequence, by the human prostate gland. J Biol Chem 266:2499–2507PubMed
  30.Paweletz CP, Ornstein DK, Roth MJ, Bichsel VE, Gillespie JW, Calvert VS et al (2000) Loss of annexin 1 correlates with early onset of tumorigenesis in esophageal and prostate carcinoma. Cancer Res 60:6293–6297PubMed
  31.Patton KT, Chen HM, Joseph L, Yang XJ (2005) Decreased annexin I expression in prostatic adenocarcinoma and in high-grade prostatic intraepithelial neoplasia. Histopathology 47:597–601CrossRef PubMed
  32.Maschler S, Gebeshuber CA, Wiedemann EM, Alacakaptan M, Schreiber M, Custic I et al (2010) Annexin A1 attenuates EMT and metastatic potential in breast cancer. EMBO Mol Med 2:401–414. doi:10.​1002/​emmm.​201000095 PubMedCentral CrossRef PubMed
  33.Liao Q, Kleeff J, Xiao Y, Guweidhi A, Schambony A, Töpfer-Petersen E et al (2003) Preferential expression of cystein-rich secretory protein-3 (CRISP-3) in chronic pancreatitis. Histol Histopathol 18:425–433PubMed
  34.Tapinos NI, Polihronis M, Thyphronitis G, Moutsopoulos HM (2002) Characterization of the cysteine-rich secretory protein 3 gene as an early-transcribed gene with a putative role in the pathophysiology of Sjögren’s syndrome. Arthritis Rheum 46:215–222CrossRef PubMed
  
• 作者单位：Bhakti R. Pathak (1)
  Ananya A. Breed (1)
  Snehal Apte (1)
  Kshitish Acharya (2) (3)
  Smita D. Mahale (1)
  
  1. Division of Structural Biology, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Jehangir Merwanji Street, Parel, Mumbai, 400012, India
  2. Institute of Bioinformatics and Applied Biotechnology (IBAB), Biotech Park, Bengaluru, India
  3. Shodhaka Life Sciences, Pvt. Ltd., IBAB, Bengaluru, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

Cysteine-rich secretory protein 3 (CRISP-3) is upregulated in prostate cancer as compared to the normal prostate tissue. Higher expression of CRISP-3 has been linked to poor prognosis and hence it has been thought to act as a prognostic marker for prostate cancer. It is proposed to have a role in innate immunity but its role in prostate cancer is still unknown. In order to understand its function, its expression was stably knocked down in LNCaP cells. CRISP-3 knockdown did not affect cell viability but resulted in reduced invasiveness. Global gene expression changes upon CRISP-3 knockdown were identified by microarray analysis. Microarray data were quantitatively validated by evaluating the expression of seven candidate genes in three independent stable clones. Functional annotation of the differentially expressed genes identified cell adhesion, cell motility, and ion transport to be affected among other biological processes. Prostate-specific antigen (PSA, also known as Kallikrein 3) was the top most downregulated gene whose expression was also validated at protein level. Interestingly, expression of Annexin A1 (ANXA1), a known anti-inflammatory protein, was upregulated upon CRISP-3 knockdown. Re-introduction of CRISP-3 into the knockdown clone reversed the effect on invasiveness and also led to increased PSA expression. These results suggest that overexpression of CRISP-3 in prostate tumor may maintain higher PSA expression and lower ANXA1 expression. Our data also indicate that poor prognosis associated with higher CRISP-3 expression could be due to its role in cell invasion. Keywords CRISP-3 Prostate cancer Prognosis shRNA PSA ANXA1