Molecular Markers Assisted Identification of Intraspecific Hybrids in Ziziphus mauritiana

详细信息    查看全文

• 作者：Sunil Kumar Singh (1)
  P. R. Meghwal (1)
  Rakesh Pathak (1)
  Ragini Gautam (1)
  
• 关键词：Indian jujube ; Seb ; Tikadi ; Katha ; Hybridization ; ITS ; RAPD
• 刊名：Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：84
• 期：3
• 页码：603-611
• 全文大小：434 KB
• 参考文献：1. Qu ZZ, Wang YH (1991) China fruit’s monograph—Chinese Jujube volume. China Forestry Publishing House, Beijing (in Chinese)
  2. Pareek OP (2001) Fruits for the future 2: Ber. International Centre for Underutilized Crop. Redwood Books, Wiltshire, p 290
  3. Morton J (1987) Indian jujube. In: Mortan JF, Miami FL (eds) Fruits of warm climates. Center for New Crops & Plant Products, Purdue University, Lafayette, pp 272-75. http://www.hort.purdue.edu/newcrop/morton/indianjujube.html
  4. Verheij EWM, Calabura M (1991) Plant resources of South-East Asia 2. In: Coronel RE (ed) Edible fruits and nuts. PROSEA, Pudoc, Wageningen, pp 223-25
  5. Hudina M, Liu M, Veberic R, Stampar F, Colaric M (2008) Phenolic compounds in the fruit of different varieties of Chinese jujube ( / Ziziphus jujuba Mill.). J Hort Sci Biotechnol 83:305-08
  6. Mishra T, Khullar M, Bhatia A (2011) Anticancer potential of aqueous ethanol seed extract of / Ziziphus mauritiana against cancer cell lines and Ehrlich ascites carcinoma. Evid Based Complement Altern Med. doi:10.1155/2011/765029
  7. Ndhala AR, Mupure CH, Chitindingue KM, Benhura AN, Muchuweti M (2006) Antioxidant potentials and degree of polymerization of six wild fruits. Life Sci Res Assays 1:87-2
  8. Dahiru D, Obidoa O (2007) Pretreatment of albino rats with aqueous leaf extract of / Ziziphus mauritiana protects against alcohol induced liver damage. Trop J Pharma Res 6:705-10
  9. Bhatia A, Mishra T (2009) Free radical scavenging and antioxidant potential of / Ziziphus mauritiana (Lamk.) seed extract. J. Complement. Integra Med 8:42-6
  10. Saran PL, Godara AK, Sehrawat SK (2006) Characterization of ber ( / Ziziphus mauritiana Lamk.) genotypes. Haryana J Hort Sci 35(3/4):215-18
  11. Morton JF (2005) Indian jujube. J Expt Bot 56(422):3082-092
  12. Godara NR (1980) Studies on floral biology and compatibility behavior in ber ( / Ziziphus mauritiana Lamk.) Ph.D. thesis, Haryana Agricultural University, Hisar, India
  13. Devanshi, Singh AK, Sharma P, Singh B, Singh R, Singh NK (2007) Molecular profiling and genetic relationship among be / r ( / Ziziphus sp.) genotypes using RAPD markers. Indian J Genet 67(2):121-27
  14. Singh MP, Vashishtha BB (1984) Field screening of ber cultivars for resistance to ber fruitfly, / Carpomyia vesuviana Costa. Indian J Plant Prot 12(1):55-6
  15. Pareek OP, Nath V (1996) Ber. Coordinated fruit research in Indian Arid Zone—a two decades profiles (1976-995). National Research Centre for Arid Horticulture, Bikaner, pp 9-0
  16. Vashishtha BB, Singh MP, Singh GK, Pareek RA (1999) Breeding in Ber ( / Ziziphus mauritiana Lamk.) for the resistance of fruit fly. Abst. Fourth Agricultural Science Congress-Sustainable Agricultural Export, Jaipur, India
  17. Dhingra RP, Singh JP, Chitkara SD (1973) Varietal variations in physico-chemical characters of ber ( / Ziziphus mauritiana Lamk.). Haryana J Hort Sci 2(3/4):61-5
  18. Pareek OP, Vashishtha BB (1983) Delicious ber varieties of Rajasthan. Indian Hor 28(2):13-5
  19. Pareek OP, Vashishtha BB (1986) Vegetative and fruit characteristics of ber ( / Ziziphus mauritiana Lamk.) cultivars of Rajasthan grown in the Thar Desert. Ann Arid Zone 25(3):214-21
  20. Shukla AK, Dhandar DG, Vashishtha BB, Umamabeswari C (2004) New promising ber varieties. Indian Hort 49(3):36
  21. Cennamo P, Cafasso D (2002) Molecular markers as a tool for the identification of hybrid plane trees. Delpinoa 44:89-4
  22. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531-535 CrossRef
  23. El-Mouei R, Choumane W, Dway F (2011) Characterization and estimation of genetic diversity in Citrus rootstocks. Intl J Agric Biol 13:571-75
  24. Singh SK, Meghwal PR, Pathak R, Gautam R, Kumar S (2013) Genetic diversity in / Punica granatum revealed by nuclear rRNA, internal transcribed spacer and RAPD Polymorphism. Natl Acad Sci Lett 36(2):115-24 CrossRef
  25. Alvarez I, Wendel JF (2003) Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 29:435-55 CrossRef
  26. Carvalho A, Guedes-Pinto H, Lima BJ (2009) Genetic diversity among old Portuguese bread wheat cultivars and botanical varieties evaluated by ITS rDNA PCR-RFLP markers. J Genet 88(3):363-67 CrossRef
  27. Xiao LQ, Moller M, Zhu H (2010) High nrDNA ITS polymorphism in the ancient extant seed plant cycas: incomplete concerted evolution and the origin of pseudogenes. Mol Phylogenet Evol 55:168-77 CrossRef
  28. Singh AK, Devanshi S, Sharma P, Singh R, Singh B, Koundal KR, Singh NK (2007) Assessment of genetic diversity in / Ziziphus mauritiana using inter-simple sequence repeat markers. J Plant Biochem Biotechnol 16:35-0 CrossRef
  29. Kakani RK, Singh SK, Pancholy A, Meena RS, Pathak R, Raturi A (2011) Assessment of genetic diversity in / Trigonella foenum- / graecum based on nuclear ribosomal DNA, internal transcribed spacer and RAPD analysis. Plant Mol Bio Rep 29:315-23 CrossRef
  30. Raturi A, Singh SK, Sharma V, Pathak R (2012) Molecular characterization of / Vigna radiata (L.) Wilczek genotypes based on nuclear ribosomal DNA and RAPD polymorphism. Mol Biol Rep 39(3):2455-465 CrossRef
  31. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315-22
  32. Perrière G, Gouy M (1996) WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78:364-69 CrossRef
  33. Sneath PHA, Sokal RR (1973) Numerical taxonomy. W. H. Freeman, San Francisco
  34. Rohlf FJ (1997) NTSYS pc: numerical taxonomy and multivariate analysis system version 2.02h. Exeter software, New York
  35. Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchell SE, Kresovich S, Zeigle J (1997) An evaluation of the utility of SSR loci as molecular markers in maize ( / Zea mays L.): comparison with data from RFLPs and pedigree. Theor Appl Genet 95:163-73 CrossRef
  36. Khan H, Sivalingam PN, Chauhan S, Awasthi OP, More TA (2013) Improved crossing technique and identification of true F1 hybrids of / Ziziphus mauritiana Lam. by molecular markers. Sci Hortic 150:164-71 CrossRef
  37. Rieserberg LH, Carted R, Zona S (1990) Molecular test of hypothesized hybrid origin of two diploid / Helianthus species (Asteraceae). Evolution 44:1498-511 CrossRef
  38. Kanzaki S, Sugivra A, Yonemori K, Utsunomiya N, Subhadrabandhu S (1997) Chemotaxonomic studies on some Diospyros species by flavonoids compositions in leaves. In: Subhadrabandhu S (Ed) Proceedings of the first international persimmon symposium, Chiang mai, Thailand, 17-9 July 1996. Acta Hortic 436:93-9
  39. Williams CA, Harborne JB, Newman M, GreenHam J, Eagles J (1997) Chrysin and other leaf exudates flavonoids in the genus Pelargonium. Phytochemistry 46:1349-353 CrossRef
  40. Robards K, Antolovich M, Boyd S (1997) Characterisation of Citrus by chromatographic analysis of flavonoids. J Sci Food Agric 75(1):87-01 CrossRef
  41. Bhargava R (1983) Metabolites as indicators of polyploidy and hybridity in some bulbous angiosperms. Ph.D. Thesis, University of Jammu, Jammu
  42. Bhargava R, Sharma MC, Koul AK (1986) Origin of variety Odorus Plenus of Narcissus in the light of flavonoids analysis. Nucleus 29(3):139-40
  43. Tiwari R, Baghel BS, Gupta N (2003) Morphological characters of top-worked ber cv. Banarsi. JNKVV Res J 37(2):94-6
  44. Harris SA, Ingram R (1992) Chloroplast DNA and biosystematics: the effect of intra-specific diversity and plastid transmission. Taxon 40:393-12 CrossRef
  45. Dumolin S, Demesure B, Petit RJ (1995) Inheritance of chloroplast and mitochondrial genomes in pedunculate oak investigated with an efficient PCR-based method. Theor Appl Genet 91:1253-256 CrossRef
  46. Cervera MT, Cabezas JA, Sancha JC (1998) Application of AFLP’s to the characterization of grapevine ( / Vitis vinifera L.) genetic resources: a case study with accessions from Rioja (Sapin). Theor Appl Genet 97:51-9 CrossRef
  47. Obeed RS, Harhash MM, Abdel-Mawgood AL (2008) Fruit properties and genetic diversity of five ber ( / Ziziphus mauritiana Lamk.) cultivars. Pak J Biol Sci 11(6):888-93 CrossRef
  48. Fu JX, Liu CM, Xie JH (2007) Identification and classification of ber cultivars based on ISSR and RAPD analysis. Acta Hortic 764:119-26
  49. Jing Q, Zhen D, Min MY, Ying SL, Xing ZY, Jie L, Ling WX (2009) Construction of a dense genetic linkage map and QTL analysis of trunk diameter in Chinese jujube. Sci Silvae Sin 45(8):44-9
  50. Peng JY, Shu HR, Peng SQ (2002) To address the problem of infraspecific classification of / Ziziphus jujube Mill. using RAPD data. Acta Phytotaxon Sin 40:89-4
  51. Liu P, Peng JY, Peng SQ, Zhou JY, Dai L (2005) Study on systematic relationships of / Ziziphus jujube and / Ziziphus spinosa using RAPD technique. Sci Silvae Sin 41:182-85
  52. Shasany AK, Darokar MP, Dhawan S, Gupta AK, Gupta S, Shukla AK, Patra NK, Khanuja SPS (2005) Use of RAPD and AFLP markers to identify inter and intraspecific hybrids of Mentha. J Hered 96(5):542-49 CrossRef
  53. Shoyama Y, Kawachi F, Tanaka H, Nakai R, Shibata T, Nishi K (1998) Genetic and alkaloid analysis of / Papaver species and their F1 hybrid by RAPD, HPLC and ELISA. Forensic Sci Int 91:207-17 CrossRef
  54. Lee SY, Yae BW, Kim KS (2005) Segregation patterns of several morphological characters and RAPD markers in interspecific hybrids between / Dianthus giganteus and / D. Carthusianorum. Sci Hortic 105:53-4 CrossRef
  55. Sharma PK, Bhargava R, Koul AK (1989) Studies on the flavonoids spectrum of disomic. Cytologia 54:197-02 CrossRef
  56. Bhargava R, Shukla AK, Chauhan NB, Vashishtha B, Dhandar DG (2005) Impact of hybridity on flavonoid spectrum of ber ( / Ziziphus mauritiana Lamk.). Environ Expt Bot 53:135-38 CrossRef
  57. Ma QH, Wang GX, Liang LS (2011) Development and characterization of SSR markers in Chinese jujube ( / Ziziphus jujuba Mill.) and its related species. Sci Hortic 129:597-02 CrossRef
  58. Barkley NA, Wang ML, Gillaspie AG, Dean RE, Pederson GA, Jenkins TM (2008) Discovering and verifying DNA polymorphisms in a mungbean [ / V. radiata (L.) R Wilczek] collection by Eco-Tilling and sequencing. BMC Res Notes 1:28 CrossRef
  59. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium (2013) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Aca Sci of USA 109(16):6241-246
  60. Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Gent 85:985-93 CrossRef
  61. Hernandez P, Martin A, Dorado G (1999) Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and maker-assisted selection of wheat. Mol Breed 5:245-53 CrossRef
  62. Lee GP, Lee CH, Kim CS (2004) Molecular markers derived from RAPD, SCAR and the conserved 18S rDNA sequences for classification and identification in / Pyrus pyrifolia and / P. communis. Theor Appl Gent 108:1487-491 CrossRef
  
• 作者单位：Sunil Kumar Singh (1)
  P. R. Meghwal (1)
  Rakesh Pathak (1)
  Ragini Gautam (1)
  
  1. Central Arid Zone Research Institute, Jodhpur, Rajasthan, India
  
• ISSN：2250-1746

文摘

Breeding in Ziziphus mauritiana through hybridization is limited by its small sized flowers, cross incompatibility, low fruit set and poor retention. Correct identification of a hybrid is often difficult when it resembles more with one parent or when new morphological combination of characters arise from recombination of distinct genotypes. Under present study three varieties of Z.?mauritiana viz., Tikadi, Seb and Katha were found cross compatible and resulted in improved hybrids viz., F1 (Seb?×?Tikadi), F1?×?Seb and Seb?×?Katha. Ten RAPD primers revealed a total of 108 bands in the range of 150?bp to 3?kb. Among these, 69 bands were polymorphic and exhibited 33.33-0.9?% polymorphism in the banding patterns. The dendrogram clearly delineated variety Tikadi and Katha from Seb. All the three hybrids formed a single cluster with the variety Seb. The 5.8S gene region was found to be highly conserved (99.39?%) followed by ITS-2 (97.77?%). and ITS-1 (90.6-0.8?%). Heterozygous positions in ITS sequences clearly show their hybrid nature and could perform as highly polymorphic molecular markers. Despite SNPs, INDELS and total length polymorphism among parents and their hybrids, all were molecularly identified as Z.?mauritiana and did not cause any phylogenetic error. The nucleotide polymorphism in ITS-1 region can serve as a bar code to detect and address genetic diversity within Z.?mauritiana.