Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay

详细信息    查看全文

• 作者：Yang Yang ; Xiaodong Qin ; Yingjun Sun ; Ting Chen ; Zhidong Zhang
• 关键词：Recombinase polymerase amplification ; Real ; time RT ; RPA ; HP ; PRRSV ; Molecular diagnosis ; RT ; qPCR
• 刊名：Virus Genes
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：52
• 期：6
• 页码：883-886
• 全文大小：566 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X
• 卷排序：52

文摘

A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was 70 copies of HP-PRRSV RNA/reaction. The real-time RT-RPA highly specific amplified HP-PRRSV with no cross-reaction with classic PRRSV, classic swine fever virus, pseudorabies virus, and foot-and-mouth disease virus. Assessment with 125 clinical samples showed that the developed real-time RT-RPA assay was well correlated with real-time RT-qPCR assays for detection of HP-PRRSV. These results suggest that the developed real-time RT-RPA assay is suitable for rapid detection of HP-PRRSV.