Cryptococcus gattii-Induced Infections in Dogs from Southern Brazil

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• 作者：Selwyn Arlington Headley ; Giovana Wingeter Di Santis…
• 关键词：Cryptococcosis ; Epidemiology ; Histopathology ; ITS ; type differentiation
• 刊名：Mycopathologia
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：180
• 期：3-4
• 页码：265-275
• 全文大小：1,387 KB
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• 作者单位：Selwyn Arlington Headley (1)
  Giovana Wingeter Di Santis (1)
  Brígida Kussumoto de Alcantara (2)
  Thais Correa Costa (1)
  Elisangela Olegário da Silva (1)
  Lucienne Garcia Pretto-Giordano (3)
  Lucas Alécio Gomes (4)
  Amauri Alcindo Alfieri (2)
  Ana Paula Frederico R. L. Bracarense (1)
  
  1. Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Campus Universitário, PO Box 10.011, Londrina, Paraná, 86057-970, Brazil
  2. Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
  3. Laboratory of Veterinary Mycology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
  4. Small Animal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Paraná, Brazil
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Microbial Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-0832

文摘

Cryptococcus gattii-induced cryptococcosis is an emerging infectious disease of humans and animals worldwide, with rare descriptions of this infection in domestic animals from Brazil. This study presents the findings associated with C. gattii in dogs from Londrina, Paraná, Southern Brazil. Two dogs, a 3-year-old, female German shepherd and a 6-year-old, male Boxer, were evaluated by a combination of pathological, mycological, and molecular diagnostic techniques. Significant pathological alterations included cryptococcal lymphadenitis, meningoencephalitis, tonsillitis, and rhinitis with nasal cryptococcomas in the German shepherd dog, while cryptococcal lymphadenitis and pneumonia were observed in the Boxer; both dogs had pseudocystic cryptococcosis. The mucicarmine histochemical stain readily identified the intralesional cryptococcal budding organisms in all affected tissues. Mycological culture and isolation confirmed the yeasts as C. gattii due to positive reaction with the l-canavanine glycine bromothymol blue agar. A PCR assay using the internal transcribed spacers (ITS)1 and ITS2 primers, which target the ITS1 and 2 regions including the 5.8S rRNA gene, amplified the desired amplicons; direct sequencing confirmed the isolate as C. gattii. ITS nucleotide differentiation demonstrated that the isolate forms part of the ITS type 4 Cryptococcus organisms which corresponds to the C. gattii VGII molecular subtype or the RAPD type 2 Cryptococcus organisms. Collectively, these findings confirmed the participation of C. gattii in the etiopathogenesis of the lesions observed in these dogs and expanded the epidemiological niche of this important mycotic agent to include Southern Brazil. It is noteworthy to mention that previous epidemiological studies have suggested that C. gattii-induced cryptococcosis is more frequently diagnosed in Northern relative to Southern Brazil, so these findings might suggest an expansion of the distribution of this agent within continental Brazil. Keywords Cryptococcosis Epidemiology Histopathology ITS-type differentiation