Mechanisms of omega-3 fatty acid-induced growth inhibition in MDA-MB-231 human breast cancer cells

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• 作者：Patricia D. Schley ; Humberto B. Jijon ; Lindsay E. Robinson and Catherine J. Field
• 关键词：Akt ; apoptosis ; breast cancer ; docosahexaenoic acid ; eicosapentaenoic acid ; MDA ; MB ; 231 ; NFκ ; B ; omega ; 3 fatty acid ; proliferation
• 刊名：Breast Cancer Research and Treatment
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：92
• 期：2
• 页码：187-195
• 全文大小：334 KB

文摘

The omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), inhibit the growth of human breast cancer cells in animal models and cell lines, but the mechanism by which this occurs is not well understood. In order to explore possible mechanisms for the modulation of breast cancer cell growth by omega-3 fatty acids, we examined the effects of EPA and DHA on the human breast cancer cell line MDA-MB-231. Omega-3 fatty acids (a combination of EPA and DHA) inhibited the growth of MDA-MB-231 cells by 30–40 % (ppp3H]-thymidine uptake and expression of proliferation-associated proteins (proliferating cell nuclear antigen, PCNA, and proliferation-related kinase, PRK). In addition, EPA and DHA induced apoptosis, as indicated by a loss of mitochondrial membrane potential, increased caspase activity and increased DNA fragmentation (pp