Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir

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• 作者：Patrick C. Y. Woo (1) (2) (3) (4)
  Samson S. Y. Wong (1) (2) (3) (4)
  Jade L. L. Teng (3)
  Kit-Wah Leung (3)
  Antonio H. Y. Ngan (3)
  Dong-qing Zhao (3)
  Herman Tse (1) (2) (3) (4)
  Susanna K. P. Lau (1) (2) (3) (4)
  Kwok-Yung Yuen (1) (2) (3) (4)
  
• 关键词：Leptotrichia hongkongensis ; Bacterium ; Blood culture ; Anaerobic bacteria ; Novel species ; Q93 ; R51
• 刊名：Journal of Zhejiang University SCIENCE B
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：11
• 期：6
• 页码：391-401
• 全文大小：208KB
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  21. Woo, P.C., Fung, A.M., Lau, S.K., Hon, E., Yuen, K.Y., 2002a. Diagnosis of pelvic actinomycosis by 16S ribosomal RNA gene sequencing and its clinical significance. / Diagnostic Microbiology and Infectious Disease, 43(2):113-18. [doi:10.1016/S0732-8893(02)00375-9] CrossRef
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  23. Woo, P.C., Tam, D.M., Leung, K.W., Lau, S.K., Teng, J.L., Wong, M.K., Yuen, K.Y., 2002c. / Streptococcus sinensis sp. nov., a novel species isolated from a patient with infective endocarditis. / Journal of Clinical Microbiology, 40(3):805-10. [doi:10.1128/JCM.40.3.805-810.2002] CrossRef
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  28. Woo, P.C., Tse, H., Chan, K.M., Lau, S.K., Fung, A.M., Yip, K.T., Tam, D.M., Ng, K.H., Que, T.L., Yuen, K.Y., 2004c. / Streptococcus milleri endocarditis caused by / Streptococcus anginosus. / Diagnostic Microbiology and Infectious Disease, 48(2):81-8. [doi:10.1016/j.diagmicrobio.2003.09.011] CrossRef
  29. Woo, P.C., Lau, S.K., Lin, A.W., Curreem, S.O., Fung, A.M., Yuen, K.Y., 2007. Surgical site abscess caused by / Lactobacillus fermentum identified by 16S ribosomal RNA gene sequencing. / Diagnostic Microbiology and Infectious Disease, 58(2):251-54. [doi:10.1016/j.diagmicrobio.2006.12.005] CrossRef
  30. Woo, P.C., Teng, J.L., Tsang, S.N., Tse, C.W., Lau, S.K., Yuen, K.Y., 2008. Oral cavity as natural reservoir for / Streptococcus sinensis. / Clinical Microbiology and Infection, 14(11):1075-079. [doi:10.1111/j.1469-0691.2008.02083.x] CrossRef
  31. Woo, P.C., Teng, J.L., Tsang, A.K., Tse, H., Tsang, V.Y., Chan, K.M., Lee, E.K., Chan, J.K., Ma, S.S., Tam, D.M., / et al., 2009. Development of a multi-locus sequence typing scheme for / Laribacter hongkongensis, a novel bacterium associated with freshwater fish-borne gastroenteritis and traveler’s diarrhea. / BMC Microbiology, 9(1):21. [doi:10.1186/1471-2180-9-21] CrossRef
  
• 作者单位：Patrick C. Y. Woo (1) (2) (3) (4)
  Samson S. Y. Wong (1) (2) (3) (4)
  Jade L. L. Teng (3)
  Kit-Wah Leung (3)
  Antonio H. Y. Ngan (3)
  Dong-qing Zhao (3)
  Herman Tse (1) (2) (3) (4)
  Susanna K. P. Lau (1) (2) (3) (4)
  Kwok-Yung Yuen (1) (2) (3) (4)
  
  1. State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China
  2. Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China
  3. Department of Microbiology, The University of Hong Kong, Hong Kong, China
  4. Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China
  
• ISSN：1862-1783

文摘

A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.