Levels of dioxin-like PCBs in low-volume serum samples of male patients attending fertility clinics
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- 作者：Julia Moltó ; Raiza Paul ; Nuria Ortuño…
- 关键词：Human serum ; Male infertility ; DL ; PCBs ; HRGC ; HRMS ; Pollutants ; Analytical method
- 刊名：Environmental Science and Pollution Research
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：23
- 期：4
- 页码：3463-3468
- 全文大小：829 KB
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Raiza Paul (2) (3)
Nuria Ortuño (1)
María Llanos Medrano (3) (4)
Jon Aizpurua (3) (4)
María José Gómez-Torres (2) (3)
1. Chemical Engineering Department, University of Alicante, Carretera de San Vicente s/n, P.O. Box 99, 03080, Alicante, Spain
2. Biotechnology Department, University of Alicante, Carretera de San Vicente s/n, P.O. Box 99, 03080, Alicante, Spain
3. University of Alicante, Carretera de San Vicente s/n, P.O. Box 99, 03080, Alicante, Spain
4. IVF Spain Treatment Clinic, Avenida de Ansaldo 13, 03540, Alicante, Spain - 刊物类别：Earth and Environmental Science
- 刊物主题：Environment
Environment
Atmospheric Protection, Air Quality Control and Air Pollution
Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
Industrial Pollution Prevention - 出版者：Springer Berlin / Heidelberg
- ISSN：1614-7499
文摘
An accurate and easy method for the extraction, cleanup, and HRGC-HRMS analysis of dioxin-like PCBs (DL-PCBs) in low-volume serum samples (1 mL) was developed. Serum samples were extracted several times using n-hexane and purified by acid washing. Recovery rates of labeled congeners ranged from 70 to 110 % and the limits of detection were below 1 pg/g on lipid basis. Although human studies are limited and contradictory, several studies have shown that DL-PCBs can have adverse effects on the male reproductive system. In this way, the present method was applied to 21 serum samples of male patients attending fertility clinics. The total levels obtained for the patients ranged from 6.90 to 84.1 pg WHO-TEQ/g lipid, with a mean value of 20.3 pg WHO-TEQ/g lipid. The predominant PCBs (the sum of PCB 118, 156, and 105) contributed 67 % to the mean concentration of total DL-PCBs in the samples analyzed. Keywords Human serum Male infertility DL-PCBs HRGC-HRMS Pollutants Analytical method