Knockdown of Carotenoid Cleavage Dioxygenase 4 (CCD4) via Virus-Induced Gene Silencing Confers Yellow Coloration in Peach Fruit: Evaluation of Gene Function Related to Fruit Traits
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- 作者：Songling Bai ; Pham Anh Tuan ; Miho Tatsuki…
- 关键词：Carotenoid cleavage dioxygenase 4 (CCD4) ; Peach fruits ; Transgenic fruit tress ; Tobacco rattle virus (TRV) ; Virus ; induced gene silencing (VIGS)
- 刊名：Plant Molecular Biology Reporter
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：34
- 期：1
- 页码：257-264
- 全文大小：871 KB
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Pham Anh Tuan (1)
Miho Tatsuki (1)
Hideaki Yaegaki (1)
Akemi Ohmiya (2)
Chihiro Yamamizo (2)
Takaya Moriguchi (1)
1. NARO Institute of Fruit Tree Science, 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8605, Japan
2. NARO Institute of Floricultural Science, 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8519, Japan - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Sciences
Plant Physiology - 出版者：Springer Netherlands
- ISSN：1572-9818
文摘
Transgenic approach is an excellent way for the clarification of gene function, but it is generally difficult to create transgenic plants for most of the fruit trees including peach. Even if transgenic lines are successfully obtained, it will be extremely difficult to evaluate fruit traits due to the long juvenile phase of the plants. To overcome this problem, usage of virus vector is one of the excellent approaches. In this study, we evaluated gene function related to fruit traits in peaches via virus-induced gene silencing (VIGS). Carotenoid cleavage dioxygenase 4 (CCD4) is proposed to be the key factor responsible for carotenoid degradation in white flesh peaches. Then, we knocked down the CCD4 gene in the white flesh peaches (“Akatsuki” and “Manami”) via VIGS system. Resultantly, yellow pigmentation and increased contents of carotenoids including lutein, ß-carotene, ß-cryptoxanthin, zeaxanthin, and violaxanthin were observed in the agroinfiltration portions of the white flesh peaches, which is a direct evidence that CCD4 is a determinant for yellow flesh trait of peach. Our results suggested the possible application of VIGS system for functional studies of the genes related to fruit traits. Keywords Carotenoid cleavage dioxygenase 4 (CCD4) Peach fruits Transgenic fruit tress Tobacco rattle virus (TRV) Virus-induced gene silencing (VIGS)