Use of focussed beam reflectance measurement (FBRM) for monitoring changes in biomass concentration
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- 作者：Jessica Whelan (1)
Eilis Murphy (2)
Alan Pearson (3)
Paul Jeffers (4)
Patricia Kieran (1)
Susan McDonnell (1)
Brian Glennon (1) brian.glennon@ucd.ie - 关键词：Biomass concentration – ; On ; line monitoring – ; FBRM – ; Cell morphology
- 刊名：Bioprocess and Biosystems Engineering
- 出版年：2012
- 出版时间：August 2012
- 年：2012
- 卷：35
- 期：6
- 页码：963-975
- 全文大小：479.5 KB
- 参考文献：1. Zhou WC, Hu WS (1994) Online characterization of a hybridoma cell culture process. Biotechnol Bioeng 44(2):170–177
2. Junker BH, Reddy J, Gbewonyo K, Greasham R (1994) Online and in situ monitoring technology for cell density measurement in microbial and animal cell cultures. Bioprocess Eng 10(5–6):195–207
3. Wu P, Ozturk SS, Blackie JD, Thrift JC, Figueroa C, Naveh D (1995) Evaluation and applications of optical cell density probes in mammalian cell bioreactors. Biotechnol Bioeng 45(6):495–502
4. Macmichael G, Armiger WB, Lee JF, Mutharasan R (1987) On-line measurement of hybridoma growth by culture fluorescence. Biotechnol techniques 1:213–218
5. Kilburn DG, Fitzpatrick P, Blakecoleman BC, Clarke DJ, Griffiths JB (1989) Online monitoring of cell mass in mammalian cell cultures by acoustic densitometry. Biotechnol Bioeng 33(11):1379–1384
6. Burgemeister S, Nattkemper TW, Noll T, Hoffrogge R, Flaschel E (2010) CellViCAM—Cell viability classification for animal cell cultures using dark filed micrographs. J Biotechnol 149(4):310–316
7. Guez JS, Cassar JP, Wartelle F, Dhulster P, Suhr H (2010) The viability of animal cell cultures: can it be estimated online by using in situ microscopy? Process Biochem 45(2):288–291
8. Wlaschin KF, Hu WS (2006) Fedbatch culture and dynamic nutrient feeding. Cell Culture Eng 101:43–74
9. Barrett M, McNamara M, Hao HX, Barrett P, Glennon B (2010) Supersaturation tracking for the development, optimization and control of crystallization processes. Chem Eng Res Des 88(8A):1108–1119
10. O’Grady D, Barrett M, Casey E, Glennon B (2007) The effect of mixing on the metastable zone width and nucleation kinetics in the anti-solvent crystallization of benzoic acid. Chem Eng Res Des 85(A7):945–952
11. Barrett P, Glennon B (2002) Characterizing the metastable zone width and solubility curve using Lasentec FBRM and PVM. Chem Eng Res Des 80(A7):799–805
12. Uduman N, Qi Y, Danquah MK, Hoadley AFA (2010) Marine microalgae flocculation and focused beam reflectance measurement. Chem Eng J 162(3):935–940
13. Ge XM, Zhao XQ, Bai FW (2005) Online monitoring and characterization of flocculating yeast cell flocs during continuous ethanol fermentation. Biotechnol Bioeng 90(5):523–531
14. McDonald KA, Jackman AP, Hurst S (2001) Characterization of plant suspension cultures using the focused beam reflectance technique. Biotechnol Lett 23(4):317–324
15. Pearson AP, Glennon B, Kieran PM (2004) Monitoring of cell growth using the focused beam reflectance method. J Chem Technol Biotechnol 79(10):1142–1147
16. Jeffers P, Raposo S, Lima-Costa ME, Connolly P, Glennon B, Kieran PM (2003) Focussed beam reflectance measurement (FBRM) monitoring of particle size and morphology in suspension cultures of Morinda citrifolia and Centaurea calcitrapa. Biotechnol Lett 25(23):2023–2028
17. Sparks RG, Dobbs CL (1993) The use of laser backscatter instrumentation for the online measurement of the particle size distribution of emulsions. Part Part Syst Charact 10(5):279–289
18. Hopfner T, Bluma A, Rudolph G, Lindner P, Scheper T (2010) A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring. Bioprocess Biosyst Eng 33(2):247–256
19. Pearson AP, Glennon B, Kieran PM (2003) Comparison of morphological characteristics of Streptomyces natalensis by image analysis and focused beam reflectance measurement. Biotechnol Prog 19(4):1342–1347
20. Seah SYK, Britton KL, Baker PJ, Rice DW, Asano Y, Engel PC (1995) Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis. FEBS Lett 370(1–2):93–96
21. Faulkner E, Barrett M, Okor S, Kieran P, Casey E, Paradisi F, Engel P, Glennon B (2006) Use of fed-batch cultivation for achieving high cell densities for the pilot-scale production of a recombinant protein (phenylalanine dehydrogenase) in Escherichia coli. Biotechnol Prog 22(3):889–897
22. Fitzpatrick B, O’Kennedy R (2004) The development and application of a surface plasmon resonance-based inhibition immunoassay for the determination of warfarin in plasma ultrafiltrate. J Immunol Methods 291(1–2):11–25
23. Barrett P (2002) In situ monitoring of crystallization processes. University College Dublin, Dublin
24. Heath AR, Fawell PD, Bahri PA, Swift JD (2002) Estimating average particle size by focused beam reflectance measurement (FBRM). Part Part Syst Charact 19(2):84–95
25. Emery AN, Jan DCH, Al-Rubeai M (1995) Oxygenation of intensive cell culture system. Appl Microb Biotechnol 43(6):1028–1033
26. Ishaque A, Al-Rubeai M (2004) Monitoring of apoptosis. In: Al-Rubeai M, Fussenegger M (eds) Cell engineering: apoptosis, vol 4. Springer, Heidelberg, pp 281–306
27. Van de Goor J (2004) Improvement of industrial cell culture processes by caspase-9 dominant negative and other apoptosis inhibitors. In: Al-Rubeai M, Fussenegger M (eds) Cell engineering: apoptosis, vol 4. Springer, Heidelberg, pp 211–221
28. Zeng A, Bi J (2005) Cell culture kinetics and modeling. In: Ozturk SS, Hu WS (eds) Cell culture technology for pharmaceutical and cell-based therapies. CRC Press, Boca Raton, pp 299–348
29. Franek F, Dolnikova J (1991) Hybridoma growth and monoclonal antibody production in iron-rich protein-free medium: effect of nutrient concentration. Cytotechnology 7(1):33–38
30. Mercille S, Massie B (1994) Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells. Biotechnol Bioeng 44(9):1140–1154
31. Singh RP, Al-Rubeai M, Gregory CD, Emery AN (1994) Cell death in bioreactors: a role for apoptosis. Biotechnol Bioeng 44(6):720–726
32. Bartlett M, Huang G, Larcom L, Jiang HB (2004) Measurement of particle size distribution in mammalian cells in vitro by use of polarized light spectroscopy. Appl Opt 43:1296–1307
33. Backman V, Gopal V, Kalashnikov M, Badizadegan K, Gurjar R, Wax A, Georgakoudi I, Mueller M, Boone CW, Dasari RR, Feld MS (2001) Measuring cellular structure at submicrometer scale with light scattering spectroscopy. IEEE J Sel Topics Quantum Electron 7(6):887–893
34. Gurjar RS, Backman V, Perelman LT, Georgakoudi I, Badizadegan K, Itzkan I, Dasari RR, Feld MS (2001) Imaging human epithelial properties with polarized light-scattering spectroscopy. Nat Med 7(11):1245–1248
35. Drezek R, Guillaud M, Collier T, Boiko I, Malpica A, Macaulay C, Follen M, Richards-Kortum R (2003) Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture. J Biomedical Opt 8(1):7–16
36. Collins RJ, Harmon BV, Gobe GC, Kerr JFR (1992) Inter nucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. Int J Radiat Biol 61(4):451–453 - 作者单位：1. UCD School of Chemical and Bioprocess Engineering, UCD, Belfield, Dublin 4, Ireland2. Training Department, National Institute of Bioprocessing Research and Training, Foster鈥檚 Avenue, Mount Merrion, Blackrock, Dublin 4, Ireland3. Pharmaceutical Operations, MSD Ireland, Clonmel, Tipperary, Ireland4. Process Analytical Sciences Group, Pfizer Ireland Pharmaceuticals, Loughbeg, Cork, Ireland
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Industrial Chemistry and Chemical Engineering
Industrial and Production Engineering
Waste Management and Waste Technology
Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
Food Science - 出版者：Springer Berlin / Heidelberg
- ISSN：1615-7605
文摘
The potential of focussed beam reflectance measurement (FBRM) as a tool to monitor changes in biomass concentration was investigated in a number of biological systems. The measurement technique was applied to two morphologically dissimilar plant cell suspension cultures, Morinda citrifolia and Centaurea calcitrapa, to a filamentous bacteria, Streptomyces natalensis, to high density cultures of Escherichia coli and to a murine Sp2/0 hybridoma suspension cell line, 3–2.19. In all cases, the biomass concentration proved to be correlated with total FBRM counts. The nature of the correlation varied between systems and was influenced by the concentration, nature, size and morphology of the particle under investigation.