Characterization of Pectobacterium carotovorum subsp. odoriferum causing soft rot of stored vegetables

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• 作者：M. Waleron (1)
  K. Waleron (1) (2)
  E. Lojkowska (1)
  
• 关键词：Pectobacterium carotovorum subsp. odoriferum ; recA ; rpoS ; 16S rRNA ; Chicory ; Potato ; Soft rot
• 刊名：European Journal of Plant Pathology
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：139
• 期：3
• 页码：457-469
• 全文大小：
• 参考文献：1. Alcorn, S. M., Orum, T. V., Steigerwalt, A. G., Foster, J. L. M., Fogleman, J. C., & Brenner, D. J. (1991). Taxonomy and pathogenicity of / Erwinia cacticida sp. nov. / International Journal of Systematic Bacteriology, 41, 197-12. CrossRef
  2. Darrasse, A., Priou, S., Kotoujansky, A., & Bertheau, Y. (1994). PCR and Restriction Fragment Length Polymorphism of a / pel gene as a tool to identify / Erwinia carotovora in relation to potato diseases. / Applied and Environmental Microbiology, 60, 1437-443.
  3. De Boer, S. H., & Ward, L. J. (1995). PCR detection of / Erwinia carotovora subsp. / atroseptica associated with potato tissue. / Phytopathology, 85, 854-58. CrossRef
  4. Gallelli, A., Galli, M., De Simone, D., Zaccardelli, M., & Loreti, S. (2009). Phenotypic and genetic variability of / Pectobacterium carotovorum isolated from artichoke in the Sele valley. / Journal of Plant Pathology, 91, 757-61.
  5. Gallois, A., Samson, R., Ageron, E., & Grimont, P. A. D. (1992). / Erwinia carotovora subsp. / odorifera subsp. nov., associated with odorous soft rot of chicory ( / Cichorium intybus L.). / International Journal of Systematic Bacteriology, 42, 582-88. CrossRef
  6. Gardan, L., Gouy, C., Christen, R., & Samson, R. (2003). Elevation of three subspecies of / Pectobacterium carotovorum to species level: / Pectobacterium atrosepticum sp. nov., / Pectobacterium betavasculorum sp. nov. and / Pectobacterium wasabiae sp. nov. / International Journal of Systematic and Evolutionry Microbiology, 53, 381-91. CrossRef
  7. Glasner, J. D., Marquez-Villavicencio, M., Kim, H. S., Jahn, C. E., Ma, B., Biehl, B. S., et al. (2008). Niche-specificity and the variable fraction of the / Pectobacterium pan-genome. / Molecular Plant-Microbe Interactions, 21, 1549-560. CrossRef
  8. Hauben, L., Moore, E. R. B., Vauterin, L., Steenackers, M., Mergaert, J., & Verdonck, L. (1998). Phylogenetic position of phytopathogens within the / Enterobacteriaceae. Systematic and Applied Microbiology, 21, 384-97. CrossRef
  9. Kim, H. S., Ma, B., Perna, N. T., & Charkowski, A. O. (2009). Phylogeny and virulence of naturally occurring type III secretion system-deficient / Pectobacterium strains. / Applied and Environmental Microbiology, 13, 4539-549. CrossRef
  10. Lan, W. W., Nishiwaki, Y., Akino, S., & Kondo, N. (2013). Soft rot of root chicory in Hokkaido and its causal bacteria. / Journal of General Plant Pathology, 79, 182-93. CrossRef
  11. Lojkowska, E., & Kelman, A. (1994). Comparison of the effectiveness of different methods of screening for bacterial soft rot resistance of potato tubers. / American Potato Journal, 71, 99-13. CrossRef
  12. Ma, B., Hibbing, M. E., Kim, H. S., Reedy, R. M., Yedidia, I., Breuer, J., Breuer, J., Glasner, J. D., Perna, N. T., Kelman, A., & Charkowski, A. O. (2007). The host range and molecular phylogenies of the soft rot enterobacterial genera / Pectobacterium and / Dickeya. Phytopathology, 97, 1150-163. CrossRef
  13. Nabhan, S., De Boer, S. H., Maiss, E., & Wydra, K. (2012a). Taxonomic relatedness between / Pectobacterium carotovorum subsp. / carotovorum, / Pectobacterium carotovorum subsp. / odoriferum and / Pectobacterium carotovorum subsp. / brasiliense subsp. nov. / Journal of Applied Microbiology, 113, 904-13. CrossRef
  14. Nabhan, S., Wydra, K., Linde, M., & Debener, T. (2012b). The use of two complementary DNA assays, AFLP and MLSA, for epidemic and phylogenetic studies of pectolytic enterobacterial strains with focus on the heterogeneous species / Pectobacterium carotovorum. Plant Pathology, 61, 498-08. CrossRef
  15. Nabhan, S., De Boer, S. H., Maiss, E., & Wydra, K. (2013). / Pectobacterium aroidearum sp. nov., a soft rot pathogen with preference for monocotyledonous plants. / International Journal of Systematic and Evolutionary Microbiology, 63, 2520-525. CrossRef
  16. Nassar, A., Darrasse, A., Lemattre, M., Kotoujansky, A., Dervin, C., Vedel, R., & Bertheau, Y. (1996). Characterization of / Erwinia chrysanthemi by pectinolytic isozyme polymorphism and restriction fragment length polymorphism analysis of PCR- amplified fragments of / pel genes. / Applied and Environmental Microbiology, 62, 2228-235.
  17. Nazerian, E., Sijam, K., Ahmad, Z. A. M., & Keshavarz, K. (2011). Characterization of / Pectobacterium carotovorum causing a new soft rot disease on okra in Malaysia. / Journal of General Plant Pathology, 77, 292-94. CrossRef
  18. Samson, R., Poutier, F., Sailly, M., Hingand, L., & Jouan, B. (1980). Bactéries associées ? une pourriture du collet de l’endive (in French). / Annales de phytopathologie, 12, 311-17.
  19. Schaad, N. W., Jones, J. B., & Chun, W. (2001). / Laboratory guide for identification of plant pathogenic bacteria (3rd ed.). St. Paul: APS Press.
  20. Thapa, S. P., Park, H. R., Lim, C. K., & Hur, J. H. (2011). Phylogeny of the Korean / Erwinia species as determined by comparison of 16S rDNA sequences. / Journal of Agricultural, Life and Environmental Sciences, 23, 62-9.
  21. Van Gijsegem, F., Wlodarczyk, A., Cornu, A., Reverchon, S., & Hugouvieux-Cotte-Pattat, N. (2008). Analysis of the LacI family regulators of / Erwinia chrysanthemi 3937, involvement in the bacterial phytopathogenicity. / Molecular Plant-Microbe Interactions, 21, 1471-481. CrossRef
  22. Waleron, M., Waleron, K., Podhajska, A. J., & Lojkowska, E. (2002a). Genotyping of bacteria belonging to the former / Erwinia genus by PCR-RFLP analysis of / recA gene fragment. / Microbiology, 148, 583-95.
  23. Waleron, M., Waleron, K., & ?ojkowska, E. (2002b). Genotypic characterisation of the / Erwinia genus by PCR-RFLP analysis of / rpoS Gene. / Plant Protection Science, 38, 288-90.
  24. Waleron, M., Waleron, K., Geider, K., & Lojkowska, E. (2008). Differentiation of / Erwinia amylovora and / Erwinia pyrifoliae by PCR-RFLP of / recA, / gyrA and / rpoS gene framents. / European Journal of Plant Pathology, 121, 161-72. CrossRef
  25. Waleron, M., Waleron, K., & Lojkowska, E. (2013). Occurrence of / Pectobacterium wasabiae in potato field samples. / European Journal of Plant Pathology, 137, 149-58. CrossRef
  26. Yahiaoui-Zaidi, R., Ladjouzi, R., & Benallaoua, S. (2010). Pathogenic variability within biochemical groups of / Pectobacterium carotovorum isolated in Algeria from seed potato tubers. / International Journal for Biotechnology and Molecular Biology Research, 1, 1-.
  
• 作者单位：M. Waleron (1)
  K. Waleron (1) (2)
  E. Lojkowska (1)
  
  1. Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Kladki 24, 80-822, Gdansk, Poland
  2. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Gdansk, Al. Gen. Hallera 107, 80-416, Gdansk, Poland
  
• ISSN：1573-8469

文摘

Pectobacterium carotovorum subsp. odoriferum has been generally considered to have a narrow host range and has been isolated most often from chicory. Research was conducted to identify 91 Pectobacterium spp. strains isolated from different vegetables in Europe, North and South America, Asia, and Africa, and to compare their ability to cause disease in chicory and potato. Among the 91 strains, 22 strains from Europe were identified as P. c. subsp. odoriferum. Based on phylogenetic analysis of 16S rDNA, recA, and rpoS gene sequences, strains isolated from stored vegetables clustered together with the type strain of P. c. subsp. odoriferum and clustered separately from the P. c. subsp. carotovorum isolates. Eleven strains previously identified as P. c. subsp. carotovorum were reclassified as P. c. subsp. odoriferum. All P. c. subsp. odoriferum isolates were able to cause soft rot symptoms on chicory and potato. Moreover, the symptoms on potatoes were more severe at temperatures from 15 to 37?°C with P. c. subsp. odoriferum isolates than with P. atrosepticum or P. c. subsp. carotovorum isolates. Tissue maceration by P. c. subsp. odoriferum isolates was highest at 28?°C, and at that temperature tissue maceration was two-times greater for P. c. subsp. odoriferum isolates than for P. c. subsp. carotovorum isolates. Symptoms on inoculated chicory leaves were more severe with P. c. subsp. odoriferum (regardless of origin) than with other subspecies or species. To our knowledge, this is the first report that P. c. subsp. odoriferum occurs on a wide range of vegetables and has the ability to cause soft rot during potato storage.