Plant Pathogenic Microbial Communication Affected by Elevated Temperature in Pectobacterium carotovorum subsp. carotovorum

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• 作者：N. D. Saha ; A. Chaudhary ; S. D. Singh ; D. Singh ; S. Walia…
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：71
• 期：5
• 页码：585-593
• 全文大小：1,361 KB
• 参考文献：1.Bender CL, Alarco′n-Chaidez F, Gross DC (1999) Pseudomonas syringae phytotoxins: mode of action, regulation, and biosynthesis by peptide and polyketide synthetases. Microbiol Mol Biol Rev 63:266-92PubMed Central PubMed
  2.Blosser RS, Gray KM (2000) Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for Nacyl homoserine lactone autoinducers. J Microbiol Meth 40:47-5CrossRef
  3.Byers JT, Lucas C, George PC, Welch M (2002) Nonenzymatic Turnover of an Erwinia carotovora Quorum-Sensing Signaling Molecule. J Bacteriol 184:1163-171PubMed Central CrossRef PubMed
  4.Chatterjee A, Cui YY, Liu Y, Dumenyo CK, Chatterjee AK (1995) Inactivation of rsmA leads to overproduction of extracellular pectinases, cellulases, and proteases in Erwinia carotovora subsp. carotovora in the absence of the starvation/cell density sensing signal, N-(3-oxohexanoyl)-?~?-homoserine lactone. Appl Environ Microbiol 61:1959-967PubMed Central PubMed
  5.Eberhard A, Burlingame AL, Eberhard C, Kenyon GL, Nealson KH, Oppenheimer NJ (1981) Structural identification of auto inducer of Photobacterium fischeri luciferase. Biochemistry 20(1981):2444-449CrossRef PubMed
  6.Hasegawa H, Chatterjee A, Cui Y, Chatterjee AK (2005) Elevated Temperature Enhances Virulence of Erwinia carotovora subsp. carotovora Strain EC153 to plants and stimulates production of the quorum sensing signal, N-Acyl homoserine lactone, and extracellular proteins. Appl Environ Microbiol 71:4655-663PubMed Central CrossRef PubMed
  7.Jones S, Yu B, Bainton NJ, Birdsall M, Bycroft BW, Chhabra SR, Cox AJR, Golby P, Reeves PJ, Stephens S, Winson MK, Salmond GPC, Stewart GSAB, Williams P (1993) The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa. EMBO J 12:2477-482PubMed Central PubMed
  8.Nasser W, Bouillant ML, Salmond G, Reverchon S (1998) Characterization of the Erwinia chrysanthemi expI–expR locus directing the synthesis of two N-acylhomoserine lactone signal molecules. Mol Microbiol 29:1391-405CrossRef PubMed
  9.Nuske J, Fritsche W (1989) Phaseolotoxin production by Pseudomonas syringae pv. phaseolicola: the influence of temperature. J Basic Microbiol 29:441-47CrossRef PubMed
  10.Palmer DA, Bender CL (1993) Effects of environmental and nutritional factors on production of the polyketide phytotoxin coronatine by Pseudomonas syringae pv. glycinea. Appl Environ Microbiol 59:1619-626PubMed Central PubMed
  11.Pirhonen M, Flego D, Heikinheimo R, Palva ET (1993) A small diffusible molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. EMBO J 12:2467-476PubMed Central PubMed
  12.Rohde BH, Pohlack B, Ullrich MS (1998) Occurrence of thermoregulation of genes involved in coronatine biosynthesis among various Pseudomonas syringae strains. J Basic Microbiol 38:41-0CrossRef PubMed
  13.Sezgin E, Karclolu A, Esentepe M, Onan E (1984) Studies on determination of diseases on ornamental plants as grown commercially in Aegean Region. Plant Protection Research Institute, Project No. 1051023/1 Bornova, Izmir, Turkey (Turkish, with English abstract)
  14.Shaw PD, Ping G, Daly SL, Cha C, Cronan JE, Rinehart KL, Farrand SK (1997) Detecting and characterizing N-acylhomoserine lactone signal molecules by thin-layer chromatography. Proc Nat Acad Sci USA 94:6036-041PubMed Central CrossRef PubMed
  15.Straley SC, Skrzypek E, Plano GV, Bliska JB (1993) Yops of Yersinia spp. pathogenic for humans. Infect Immun 61:105-110
  16.Whitehead NA, Barnard AML, Slater H, Simpson NJL, Salmond GPC (2001) Quorum sensing in Gram-negative bacteria. FEMS Microbiol Rev 25:365-04CrossRef PubMed
  
• 作者单位：N. D. Saha (1)
  A. Chaudhary (1)
  S. D. Singh (1)
  D. Singh (2)
  S. Walia (3)
  T. K. Das (4)
  
  1. Centre for Environment Science and Climate Resilient Agriculture (CESCRA), Indian Agricultural Research Institute (IARI), New Delhi, 110012, India
  2. Division of Plant Pathology, Indian Agricultural Research Institute (IARI), New Delhi, India
  3. Division of Agricultural Chemicals, Indian Agricultural Research Institute (IARI), New Delhi, India
  4. Division of Agronomy, Indian Agricultural Research Institute (IARI), New Delhi, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Gram-negative plant pathogenic bacteria regulate specific gene expression in a population density-dependent manner by sensing level of Acyl-Homoserine Lactone (HSL) molecules which they produce and liberate to the environment, called Quorum Sensing (QS). The production of virulence factors (extracellular enzyme viz. cellulase, pectinase, etc.) in Pectobacterium carotovorum subsp. carotovorum (Pcc) is under strong regulation of QS. The QS signal molecule, N-(3-oxohexanoyl)-l-Homoserine Lactone (OHHL) was found as the central regulatory system for the virulence factor production in Pcc and is also under strict regulation of external environmental temperature. Under seven different incubation temperatures (24, 26, 28, 30, 33, 35, and 37 °C) in laboratory condition, highest amount of OHHL (804 violacein unit) and highest (79 %) Disease Severity Index (DSI) were measured at 33 °C. The OHHL production kinetics showed accumulation of highest concentration of OHHL at late log phase of the growth but diminution in the concentration occurred during stationary phase onwards to death phase. At higher temperature (35 and 37 °C) exposure, OHHL was not at detectable range. The effect of temperature on virulence factor production is the concomitant effect of HSL production and degradation which justifies less disease severity index in cross-inoculated tomato fruits incubated at 35 and 37 °C. The nondetection of the OHHL in the elevated temperature may because of degradation as these signal molecules are quite sensitive and prone to get degraded under different physical factors. This result provides the rationale behind the highest disease severity up to certain elevated temperature and leaves opportunities for investigation on mutation, co-evolution of superior plant pathogen with more stable HSL signals-mediated pathogenesis under global warming context.