Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices
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- 作者：Juan de Dios Caballero (1) (2)
Rosa del Campo (1) (2)
Marta Tato (1) (2)
Elia G贸mez G de la Pedrosa (1) (2)
Marta Cobo (1)
Carla L贸pez-Causap茅 (2) (3)
Enrique G贸mez-Mampaso (1)
Antonio Oliver (2) (3)
Rafael Cant贸n (1) (2)
Spanish Network for Cystic Fibrosis Microbiology Laboratories
1. Servicio de Microbiolog铆a ; Hospital Universitario Ram贸n y Cajal and Instituto Ram贸n y Cajal de Investigaciones Sanitarias (IRYCIS) ; Madrid ; 28034 ; Spain
2. Red Espa帽ola de Investigaci贸n en Patolog铆a Infecciosa (REIPI) ; Madrid ; Spain
3. Servicio de Microbiolog铆a y Unidad de Investigaci贸n ; Hospital Universitario Son Espases ; Instituto de Investigaci贸n Sanitaria de Palma (IdISPa) ; Palma de Mallorca ; Spain - 关键词：Cystic fibrosis ; Microbiology ; Laboratory procedures ; Respiratory samples ; Consensus guidelines
- 刊名：BMC Microbiology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：965 KB
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- 出版者：BioMed Central
- ISSN：1471-2180
文摘
Background The microbiological procedures for cystic fibrosis (CF) samples of 17 participating Spanish centers were examined to verify their compliance with current international and national guidelines and to implement the best standards of care for microbiology practices. A 47-item questionnaire covering different CF microbiology aspects was sent to participant laboratories. Telephone interviews were performed when necessary. Data about samples processing for bacteria, mycobacteria and fungi were collected. Results Gene sequencing (71%), MALDI-TOF (59%) or both (94%) were available for most laboratories. Susceptibility testing was performed by automated microdilution systems (94%) and manual diffusion methods (59%). However, a low use of selective media for Staphylococcus aureus (59%) and Burkholderia cepacia complex (71%), and of epidemiological typing methods (41%) was reported. Conclusions Most Spanish laboratories are in agreement with consensus guidelines for the processing of CF respiratory samples, but need to improve in the use of specific selective media and typing methods for epidemiologic studies.