Synergistic and sequential effects of BMP-2, bFGF and VEGF on osteogenic differentiation of rat osteoblasts

详细信息    查看全文

• 作者：Peipei Li (1)
  Yan Bai (1)
  Guangfu Yin (1)
  Ximing Pu (1)
  Zhongbing Huang (1)
  Xiaoming Liao (1)
  Xianchun Chen (1)
  Yadong Yao (1)
  
• 关键词：Vascular endothelial growth factor (VEGF) ; Bone morphogenetic protein ; 2 (BMP ; 2) ; Basic fibroblast growth factor (bFGF) ; Synergistic effect ; Sequential effect
• 刊名：Journal of Bone and Mineral Metabolism
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：32
• 期：6
• 页码：627-635
• 全文大小：1,698 KB
• 参考文献：1. Zhao Z, Zhao M, Xiao G, Franceschi RT (2005) Gene transfer of the Runx2 transcription factor enhances osteogenic activity of bone marrow stromal cells in vitro and in vivo. Mol Ther 12:247鈥?53 CrossRef
  2. Fu Y-C, Nie H, Ho M-L, Wang C-K, Wang C-H (2008) Optimized bone regeneration based on sustained release from three-dimensional fibrous PLGA/HAp composite scaffolds loaded with BMP-2. Biotechnol Bioeng 99:996鈥?006 CrossRef
  3. Nie H, Soh BW, Fu Y-C, Wang C-H (2008) Three-dimensional fibrous PLGA/HAp composite scaffold for BMP-2 delivery. Biotechnol Bioeng 99:223鈥?34 CrossRef
  4. Kempen DHR, Lu L, Heijink A, Hefferan TE, Creemers LB, Maran A, Yaszemski MJ, Dhert WJA (2009) Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration. Biomaterials 30:2816鈥?825 CrossRef
  5. Deckers MM, Karperien M, van der Bent C, Yamashita T, Papapoulos SE, L枚wik CW (2000) Expression of vascular endothelial growth factors and their receptors during osteoblast differentiation. Endocrinology 141:1667鈥?674 CrossRef
  6. Grasselli F, Basini G, Bussolati S, Tamanini C (2002) Effects of VEGF and bFGF on proliferation and production of steroids and nitric oxide in porcine granulosa cells. Reprod Domest Anim 37:362鈥?68 CrossRef
  7. Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E, Takagishi K, Kato Y (2001) Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun 288:413鈥?19 CrossRef
  8. Fujimura K, Bessho K, Okubo Y, Kusumoto K, Segami N, Lizuka T (2002) The effect of fibroblast growth factor-2 on the osteoinductive activity of recombinant human bone morphogenetic protein-2 in rat muscle. Arch Oral Biol 47:577鈥?84 CrossRef
  9. Chaudhary LR, Hofmeister AM, Hruska KA (2004) Differential growth factor control of bone formation through osteoprogenitor differentiation. Bone 34:402鈥?11 CrossRef
  10. Nishimori S, Tanaka Y, Chiba T, Fujii M, Imamura T, Miyazono K, Ogasawara T, Kawaguchi H, Igarashi T, Fujita T, Tanaka K, Toyoshima H (2001) Smad-mediated transcription is required for transforming growth factor-尾1-induced p57Kip2 proteolysis in osteoblastic cells. J Biol Chem 276:10700鈥?0705 CrossRef
  11. Song I, Kim B-S, Kim C-S, Im G-I (2011) Effects of BMP-2 and vitamin D3 on the osteogenic differentiation of adipose stem cells. Biochem Biophys Res Co 408:126鈥?31 CrossRef
  12. Camozzi V, Tossi A, Simoni E, Pagani F, Francucci CM, Moro L (2007) Role of biochemical markers of bone remodeling in clinical practice. J Endocrinol Invest 30:13鈥?7 CrossRef
  13. Blanco Calvo M, Bol贸s Fern谩ndez V, Medina Villaamil V, Aparicio Gallego G, D铆az Prado S, Grande Pulido E (2009) Biology of BMP signaling and cancer. Clin Transl Oncol 11:126鈥?37 CrossRef
  14. Miyazono K, Maeda S, Imamura T (2005) BMP receptor signaling: transcriptional targets regulation of signals, and signaling cross-talk. Cytokine Growth Factor Rev 16:251鈥?63 CrossRef
  15. Ryoo HM, Lee MH, Kim YJ (2006) Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cells. Gene 366:51鈥?7 CrossRef
  16. Peng H, Usas A, Olshanski A, Ho AM, Gearhart B, Cooper GM, Huard J (2005) VEGF improves, whereas sFlt1 inhibits, BMP2-induced bone formation and bone healing through modulation of angiogenesis. J Bone Miner Res 20:2017鈥?027 CrossRef
  17. Celil AB, Campbell PG (2005) BMP-2 and insulin-like growth factor-I mediate Osterix (Osx) expression in human mesenchymal stem cells via the MAPK and protein kinase D signaling pathways. J Biol Chem 280:31353鈥?1359 CrossRef
  18. Deschaseaux F, Sens茅b茅e L, Heymann D (2009) Mechanisms of bone repair and regeneration. Trends Mol Med 15:417鈥?29 CrossRef
  19. Maegawa N, Kawamura K, Hirose M, Yajima H, Takakura Y, Ohgushi H (2007) Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2). J Tissue Eng Regen Med 1:306鈥?13 CrossRef
  20. Fakhry A, Ratisoontorn C, Vedhachalam C, Salhab I, Koyama E, Leboy P, Pacifici M, Kirschner RE, Nah H-D (2005) Effects of FGF-2/-9 in calvarial bone cell cultures: differentiation stage-dependent mitogenic effect, inverse regulation of BMP-2 and noggin, and enhancement of osteogenic potential. Bone 36:254鈥?66 CrossRef
  
• 作者单位：Peipei Li (1)
  Yan Bai (1)
  Guangfu Yin (1)
  Ximing Pu (1)
  Zhongbing Huang (1)
  Xiaoming Liao (1)
  Xianchun Chen (1)
  Yadong Yao (1)
  
  1. College of Materials Science and Engineering, Sichuan University, Chengdu, 610064, People鈥檚 Republic of China
  
• ISSN：1435-5604

文摘

In the present study, the effects of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on regulation of rat osteoblast (ROB) maturation in vitro were investigated. It was found that the proliferation, differentiation and mineralization of ROBs were all dose-dependently increased at particular times in the case of treatment with only one growth factor. To investigate the effects of combined treatment, ROBs were treated with either a single application of a relatively high dose of each growth factor, or binary/triple combined applications of relatively low doses of the growth factors. Osteogenic differentiation was significantly promoted in the triple combination treatment of BMP-2, VEGF and bFGF compared with the single or binary combination treatments. The optimal timing of the triple combination to enhance osteogenesis was also tested. When bFGF and VEGF were added in the early stage, and BMP-2 and VEGF were added in the late stage, osteogenic differentiation of ROBs could be enhanced more effectively. These results could be used to construct bone tissue engineering scaffolds that release growth factors sequentially.