Inhibition of Aspergillus parasiticus and cancer cells by marine actinomycete strains
详细信息 查看全文
- 作者：Ping Li (1)
Peisheng Yan (1) (2) - 关键词：actinomycetes ; antifungal ; antitumor ; tip culture method ; MTT ; colony formation assay
- 刊名：Journal of Ocean University of China
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：13
- 期：6
- 页码：985-994
- 全文大小：939 KB
- 参考文献：1. Asolkar, R. N., Kirkland, T. N., Jensen, P. R., and Fenical, W., 2010. Arenimycin, an antibiotic effective against rifampin- and methicillin-resistant / Staphylococcus aureus from the marine actinomycete / Salinispora arenicola. / Journal of Antibiotics ( / Tokyo), 63(1): 37-9. CrossRef
2. Bérdy, J., 2005. Bioactive microbial metabolites. / Journal of Antibiotics ( / Tokyo), 58(1): 1-6, DOI: 10.1038/ja.2005.1. CrossRef
3. Bode, H. B., Bethe, B., H?fs, R., and Zeeck, A., 2002. Big effects from small changes: possible ways to explore nature’s chemical diversity. / Chembiochem, 3(7): 619-27, DOI: 10.1002/1439-7633. CrossRef
4. Bredholt, H., Fjaervik, E., Johnsen, G., and Zotchev, S. B., 2008. Actinomycetes from sediments in the Trondheim fjord, Norway: Diversity and biological activity. / Marine Drugs, 6(1): 12-4, DOI: 10.3390/md6010012. CrossRef
5. Bull, A. T., and Stach, J. E., 2007. Marine actinobacteria: new opportunities for natural product search and discovery. / Trends in Microbiology, 15(11): 491-99, DOI: 10.1016/j.tim.2007.10.004] CrossRef
6. Carlson, S., Marler, L., Nam, S. J., Santarsiero, B. D., Pezzuto, J. M., and Murphy, B. T., 2013. Potential chemopreventive activity of a new macrolide antibiotic from a marine-derived / Micromonospora sp.. / Marine Drugs, 11(4): 1152-161, DOI: 10.3390/md11041152. CrossRef
7. Charoensopharat, K., Thummbenjapone, P., Sirithorn, P., and Thammasirirak, S., 2008. Antibacterial substance produced by / Streptomyces sp. No 87. / African Journal of Biotechnology, 7(9): 1362-368, DOI: 10.5897/AJB08.017.
8. Dharmaraj, S., 2010. Marine / Streptomyces as a novel source of bioactive substances. / World Journal of Microbiology and Biotechnology, 26(12): 2123-139, DOI: 10.1007/s11274-010-0415-6. CrossRef
9. El-Shatoury, S. A., El-Shenawy, N. S., and El-Salam, I. M. A., 2009. Antimicrobial, antitumor and in vivo cytotoxicity of actinomycetes inhabiting marine shellfish. / World Journal of Microbiology and Biotechnology, 25(9): 1547-555, DOI: 10.1007/s11274-009-0040-4. CrossRef
10. Engelhardt, K., Degnes, K. F., Kemmler, M., Bredholt, H., Fjaervik, E., Klinkenberg, G., Sletta, H., Ellingsen, T. E., and Zotchev, S. B., 2010. Production of a new thiopeptide antibiotic, TP-1161, by a marine / Nocardiopsis species. / Applied and Environmental Microbiology, 76(15): 4969-976, DOI: 10.1128/AEM.00741-10. CrossRef
11. Fenical, W., and Jensen, P. R., 2006. Developing a new resource for drug discovery: marine actinomycete bacteria. / Nature Chemical Biology, 2(12): 666-73, DOI: 10.1038/nchem-bio841. CrossRef
12. Gao, X., Lu, Y., Xing, Y., Ma, Y., Lu, J., Bao, W., Wang, Y., and Xi, T., 2012. A novel anticancer and antifungus phenazine derivative from a marine actinomycete BM-17. / Microbiological Research, 167(10): 616-22, DOI: 10.1016/j.micres.2012.02.008. CrossRef
13. Hertweck, C., Luzhetskyy, A., Rebets, Y., and Bechthold, A., 2007. Type II polyketide synthases: Gaining a deeper insight into enzymatic teamwork. / Natural Product Reports, 24(1): 162-90, DOI: 10.1039/B507395M. Li (1)
Peisheng Yan (1) (2)
1. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150001, P. R. China
2. School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, P. R. China - ISSN：1993-5021
文摘
Ten actinomycete strains isolated from the Yellow Sea off China’s coasts were identified as belonging to two genera by 16S rDNA phylogenetic analysis: Streptomyces and Nocardiopsis. Six Streptomyces strains (MA10, 2SHXF01-3, MA35, MA05-2, MA05-2-1 and MA08-1) and one Nocardiopsis strain (MA03) were predicted to have the potential to produce aromatic polyketides based on the analysis of the KSα (ketoacyl-synthase) gene in the type II PKS (polyketides synthase) gene cluster. Four strains (MA03, MA01, MA10 and MA05-2) exhibited significant inhibitory effects on mycelia growth (inhibition rate >50%) and subsequent aflatoxin production (inhibition rate >75%) of the mutant aflatoxigenic Aspergillus parasiticus NFRI-95. The ethyl acetate extracts of the broth of these four strains displayed significant inhibitory effects on mycelia growth, and the IC50 values were calculated (MA03: 0.275 mg mL?, MA01: 0.106 mg mL?, MA10: 1.345 mg mL? and MA05-2: 1.362 mg mL?). Five strains (2SHXF01-3, MA03, MA05-2, MA01 and MA08-1) were selected based on their high cytotoxic activities. The ethyl acetate extract of the Nocardiopsis strain MA03 was particularly noted for its high antitumor activity against human carcinomas of the cervix (HeLa), lung (A549), kidney (Caki-1) and liver (HepG2) (IC50: 2.890, 1.981, 3.032 and 2.603 μg mL?, respectively). The extract also remarkably inhibited colony formation of HeLa cells at an extremely low concentration (0.5 μg mL?). This study highlights that marine-derived actinomycetes are a huge resource of compounds for the biological control of aflatoxin contamination and the development of novel drugs for human carcinomas.