Real-time PCR as a diagnostic tool for evaluating the resistance of Chinese cabbage cultivars to Verticillium wilt

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• 作者：Shuancang Yu ; Tongbing Su ; Juan Chen ; Weihong Wang…
• 关键词：Brassica rapa subsp. pekinensis ; Verticillium wilt ; V. longisporum ; Real ; time PCR
• 刊名：European Journal of Plant Pathology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：143
• 期：3
• 页码：549-557
• 全文大小：3,518 KB
• 参考文献：Agrios, G. N. (2005). Plant pathology, 5th Edition
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• 作者单位：Shuancang Yu (1) (2)
  Tongbing Su (1) (2)
  Juan Chen (1) (2)
  Weihong Wang (1) (2)
  Fenglan Zhang (1) (2)
  Yangjun Yu (1) (2)
  Deshuang Zhang (1) (2)
  Xiuyun Zhao (1) (2)
  Guixiang Lu (1) (2)
  
  1. Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, 100097, China
  2. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, 100097, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469

文摘

Verticillium wilt disease has become increasingly serious in many regions in China over the past few years. In this study, the widespread pathogen was identified as Verticillium longisporum (V. longisporum) by mt-SSU rDNA and cytochrome b sequence analysis. By using the primer pair HW1-F/HW1-R, which was designed based on the V. longisporum-specific rDNA-ITS sequence, a real-time PCR assay was developed for rapid and specific detection of the pathogen. The real-time PCR assay enabled sensitive quantification of V. longisporum in artificial and field-infected seedlings. This study provides a method for large-scale screening of Verticillium wilt-resistant resources for Chinese cabbage breeding. Keywords Brassica rapa subsp. pekinensis Verticillium wilt V. longisporum Real-time PCR