Ultrasonic-Assisted Nebulization Extraction Coupled with SPE and HPLC for the Determination of Triterpenoids in Root of Euphorbia pekinensis Rupr.

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• 作者：Peixu Zhang (1)
  Yongri Jin (1)
  Jiafeng Chen (2)
  Hua Yao (1)
  Hanqi Zhang (1)
  Aimin Yu (1)
  Xuwen Li (1)
  
• 关键词：High performance liquid chromatography ; Ultrasonic ; assisted nebulization extraction ; Solid phase extraction ; Triterpenoids ; Euphorbia pekinensis Rupr.
• 刊名：Chromatographia
• 出版年：2013
• 出版时间：16 - August 2013
• 年：2013
• 卷：76
• 期：15
• 页码：967-974
• 全文大小：491KB
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• 作者单位：Peixu Zhang (1)
  Yongri Jin (1)
  Jiafeng Chen (2)
  Hua Yao (1)
  Hanqi Zhang (1)
  Aimin Yu (1)
  Xuwen Li (1)
  
  1. College of Chemistry, Jilin University, Changchun, 130012, People’s Republic of China
  2. Department of Neurology, The First Hospital of Jilin University, Changchun, 130012, People’s Republic of China
  

文摘

In this work, a novel method based on ultrasonic-assisted nebulization extraction coupled with solid phase extraction (UANE-SPE) and determined by high performance liquid chromatography was developed for the determination of triterpenoids in root of Euphorbia pekinensis Rupr. The experimental conditions for the UANE-SPE, such as type of extraction solvent, sample amount, type and amount of sorbent, extraction time and volume of the elution solvent, were examined and optimized. The method was successfully applied to determine euphol and tirucallol in the dried root of E. pekinensis Rupr. The recoveries of the analytes were in the range of 89.1-02.0?%. The limits of detection were 12?μg?g? for tirucallol and 10?μg?g? for euphol. The extraction yields obtained by the proposed method are higher than those obtained by the conventional extraction methods, such as reflux and ultrasonic-assisted extraction. Compared with the traditional methods, the proposed method can reduce the consumption of the labor, shorten the sample preparation time and increase the efficiencies in the extraction of active constituents from plant materials.