Lipid Accumulation by Pelletized Culture of Mucor circinelloides on Corn Stover Hydrolysate

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• 作者：Cristiano E. R. Reis (1)
  Jianguo Zhang (2)
  Bo Hu (1)
  
• 关键词：Fungal lipids ; Cell pelletization ; Hydrolysate ; Mucor circinelloides ; Growth inhibitors
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：174
• 期：1
• 页码：411-423
• 全文大小：693 KB
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• 作者单位：Cristiano E. R. Reis (1)
  Jianguo Zhang (2)
  Bo Hu (1)
  
  1. Department of Bioproducts and Biosystems Engineering, University of Minnesota, 316 BAE, 1390 Eckles Ave, Saint Paul, MN, 55108-6005, USA
  2. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China
  
• ISSN：1559-0291

文摘

Microbial oil accumulated by fungal cells is a potential feedstock for biodiesel production, and lignocellulosic materials can serve as the carbon source to support the fungal growth. The dilute acid pretreatment of corn stover can effectively break down its lignin structure, and this process generates a hydrolysate containing mostly xylose at very dilute concentration and numerous by-products that may significantly inhibit the cell growth. This study utilized corn stover hydrolysate as the culture media for the growth of Mucor circinelloides. The results showed that Mucor cells formed pellets during the cell growth, which facilitates the cell harvest from dilute solution. The results also showed that the inhibitory effect of furfural, 5-hydroxymethylfurfural (HMF), and acetic acid could be avoided if their concentration was low. In fact, all these by-products may be assimilated as carbon sources for the fungal growth. The results proved the feasibility to reuse the cultural broth water for acid pretreatment and then use for subsequent cell cultivation. The results will have a direct impact on the overall water usage of the process.